This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ryding, N. J. Articles by Champness, W. C. Search for Related Content PubMed PubMed Citation Articles by Ryding, N. J. Articles by Champness, W. C.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2002, p. 794-805, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.794-805.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System

N. Jamie Ryding,^, Todd B. Anderson,^, and Wendy C. Champness^*

Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48824

Received 21 May 2001/ Accepted 5 November 2001

The Streptomyces coelicolor absA two-component system was initially identified through analysis of mutations in the sensor kinase absA1 that caused inhibition of all four antibiotics synthesized by this strain. Previous genetic analysis had suggested that the phosphorylated form of AbsA2 acted as a negative regulator of antibiotic biosynthesis in S. coelicolor (T. B. Anderson, P. Brian, and W. C. Champness, Mol. Microbiol. 39:553–566, 2001). Genomic sequence data subsequently provided by the Sanger Centre (Cambridge, United Kingdom) revealed that absA was located within the gene cluster for production of one of the four antibiotics, calcium-dependent antibiotic (CDA). In this paper we have identified numerous transcriptional start sites within the CDA cluster and have shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. The same antibiotic-negative mutants also showed an increase in transcription from a promoter divergent to that of absA, upstream of a putative ABC transporter, in addition to an increase in transcription of absA itself. Interestingly, the negative regulation of the biosynthetic transcripts did not appear to be mediated by transcriptional regulation of cdaR (a gene encoding a homolog of the pathway-specific regulators of the act and red clusters) or by any other recognizable transcriptional regulator associated with the cluster. The role of absA in regulating the expression of the diverse antibiotic biosynthesis clusters in the genome is discussed in light of its location in the cda cluster.

Present address: Diversa Corp., San Diego, CA 92121.

Present address: Cargill Inc., Eddyville, IA 52553.

This article has been cited by other articles:

• Bonner, C. A., Disz, T., Hwang, K., Song, J., Vonstein, V., Overbeek, R., Jensen, R. A. (2008). Cohesion Group Approach for Evolutionary Analysis of TyrA, a Protein Family with Wide-Ranging Substrate Specificities. Microbiol. Mol. Biol. Rev. 72: 13-53 [Abstract] [Full Text]  
• McArthur, M., Bibb, M. J. (2008). Manipulating and understanding antibiotic production in Streptomyces coelicolor A3(2) with decoy oligonucleotides. Proc. Natl. Acad. Sci. USA 105: 1020-1025 [Abstract] [Full Text]  
• McKenzie, N. L., Nodwell, J. R. (2007). Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters. J. Bacteriol. 189: 5284-5292 [Abstract] [Full Text]  
• Soule, T., Stout, V., Swingley, W. D., Meeks, J. C., Garcia-Pichel, F. (2007). Molecular Genetics and Genomic Analysis of Scytonemin Biosynthesis in Nostoc punctiforme ATCC 29133. J. Bacteriol. 189: 4465-4472 [Abstract] [Full Text]  
• Li, W., Ying, X., Guo, Y., Yu, Z., Zhou, X., Deng, Z., Kieser, H., Chater, K. F., Tao, M. (2006). Identification of a Gene Negatively Affecting Antibiotic Production and Morphological Differentiation in Streptomyces coelicolor A3(2). J. Bacteriol. 188: 8368-8375 [Abstract] [Full Text]  
• McArthur, M., Bibb, M. (2006). In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression: implications for bacterial chromosome structure. Nucleic Acids Res 34: 5395-5401 [Abstract] [Full Text]  
• Gal, M.-F. C.-L., Thurston, L., Rich, P., Miao, V., Baltz, R. H. (2006). Complementation of daptomycin dptA and dptD deletion mutations in trans and production of hybrid lipopeptide antibiotics.. Microbiology 152: 2993-3001 [Abstract] [Full Text]  
• Bihlmaier, C., Welle, E., Hofmann, C., Welzel, K., Vente, A., Breitling, E., Muller, M., Glaser, S., Bechthold, A. (2006). Biosynthetic Gene Cluster for the Polyenoyltetramic Acid {alpha}-Lipomycin.. Antimicrob. Agents Chemother. 50: 2113-2121 [Abstract] [Full Text]  
• Heinzelmann, E., Berger, S., Muller, C., Hartner, T., Poralla, K., Wohlleben, W., Schwartz, D. (2005). An acyl-CoA dehydrogenase is involved in the formation of the {Delta}cis3 double bond in the acyl residue of the lipopeptide antibiotic friulimicin in Actinoplanes friuliensis. Microbiology 151: 1963-1974 [Abstract] [Full Text]  
• Sheeler, N. L., MacMillan, S. V., Nodwell, J. R. (2005). Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor. J. Bacteriol. 187: 687-696 [Abstract] [Full Text]  
• Xie, G., Keyhani, N. O., Bonner, C. A., Jensen, R. A. (2003). Ancient Origin of the Tryptophan Operon and the Dynamics of Evolutionary Change. Microbiol. Mol. Biol. Rev. 67: 303-342 [Abstract] [Full Text]