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Journal of Bacteriology, February 2002, p. 846-848, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.846-848.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Menaquinones in Fe(III) Reduction by Membrane Fractions of Shewanella putrefaciens

Daad A. Saffarini,1* Seth L. Blumerman,2 and Karen J. Mansoorabadi1

Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53211,1 University of Massachusetts, Amherst, Massachusetts 010032

Received 15 June 2001/ Accepted 31 October 2001

Two Tn5-generated mutants of Shewanella putrefaciens with insertions in menD and menB were isolated and analyzed. Both mutants were deficient in the use of several terminal electron acceptors, including Fe(III). This deficiency was overcome by the addition of menaquinone (vitamin K2). Isolated membrane fractions from both mutants were unable to reduce Fe(III) in the absence of added menaquinone when formate was used as the electron donor. These results indicate that menaquinones are essential components for the reduction of Fe(III) by both whole cells and purified membrane fractions when formate or lactate is used as the electron donor.

* Corresponding author. Mailing address: University of Wisconsin-Milwaukee, Department of Biological Sciences, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-4214. Fax: (414) 229-3926. E-mail: daads{at}uwm.edu.

Journal of Bacteriology, February 2002, p. 846-848, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.846-848.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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