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Journal of Bacteriology, February 2002, p. 853-856, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.853-856.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evidence for a Fourth Hydrogenase in Desulfovibrio fructosovorans

Laurence Casalot,^1,2 Gilles De Luca,³ Zorah Dermoun,^1,4 Marc Rousset,¹ and Pascale de Philip^1,4*

Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036-CNRS, 13402 Marseille Cedex 09,¹ Département d’Ecophysiologie Végétale et de Microbiologie, CEA/Cadarache, 13108 Saint-Paul-lez-Durance Cedex,³ Université de Provence, 13331 Marseille Cedex 03,⁴ Laboratoire de Microbiologie, IRD, ESIL, 13288 Marseille Cedex 09, France²

Received 9 April 2001/ Accepted 24 October 2001

A strain devoid of the three hydrogenases characterized for Desulfovibrio fructosovorans was constructed using marker exchange mutagenesis. As expected, the H₂-dependent methyl viologen reduction activity of the strain was null, but physiological studies showed no striking differences between the mutated and wild-type strains. The H⁺-D₂ exchange activity measured in the mutated strain indicates the presence of a fourth hydrogenase in D. fructosovorans.

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* Corresponding author. Mailing address: BIP-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 09, France. Phone: 33 (0)4 91 16 43 40. Fax: 33 (0)4 91 71 33 21. E-mail: dephilip{at}ibsm.cnrs-mrs.fr.

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Journal of Bacteriology, February 2002, p. 853-856, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.853-856.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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