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Journal of Bacteriology, February 2002, p. 861-863, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.861-863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

NADPH-Dependent L-Sorbose Reductase Is Responsible for L-Sorbose Assimilation in Gluconobacter suboxydans IFO 3291

Masako Shinjoh,* Masaaki Tazoe, and Tatsuo Hoshino

Department of Applied Microbiology, Nippon Roche Research Center, Kamakura 247, Japan

Received 22 June 2001/ Accepted 31 October 2001

The NADPH-dependent L-sorbose reductase (SR) of L-sorbose-producing Gluconobacter suboxydans IFO 3291 contributes to intracellular L-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced L-sorbose, indicating that the SR functions mainly as an L-sorbose-reducing enzyme in vivo and not as a D-sorbitol-oxidizing enzyme.


* Corresponding author. Mailing address: Department of Applied Microbiology, Nippon Roche Research Center, 200 Kajiwara, Kamakura 247, Japan. Phone: 81-467-47-2226. Fax: 81-467-45-6812. E-mail: masako.shinjoh{at}roche.com.


Journal of Bacteriology, February 2002, p. 861-863, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.861-863.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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