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Journal of Bacteriology, February 2002, p. 864-866, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.864-866.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

ZupT Is a Zn(II) Uptake System in Escherichia coli

Gregor Grass,1 Marco D. Wong,2 Barry P. Rosen,2 Ron L. Smith,3 and Christopher Rensing1*

Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 85718,1 Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 48201,2 Department of Biology, University of Texas at Arlington, Arlington, Texas 760193

Received 22 August 2001/ Accepted 30 October 2001

Escherichia coli zupT (ygiE), encoding a ZIP family member, mediated zinc uptake. Growth of cells disrupted in both zupT and the znuABC operon was inhibited by EDTA at a much lower concentration than a single mutant or the wild type. Cells expressing ZupT from a plasmid exhibited increased uptake of 65Zn2+.

* Corresponding author. Mailing address: Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bldg. no. 38, Rm. 429, Tucson, AZ 85721. Phone: (520) 626-8482. Fax: (520) 621-1647. E-mail: rensingc{at}ag.arizona.edu.

Journal of Bacteriology, February 2002, p. 864-866, Vol. 184, No. 3
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.3.864-866.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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