This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heeb, S.
Right arrow Articles by Haas, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heeb, S.
Right arrow Articles by Haas, D.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2002, p. 1046-1056, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1046-1056.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulatory RNA as Mediator in GacA/RsmA-Dependent Global Control of Exoproduct Formation in Pseudomonas fluorescens CHA0

Stephan Heeb, Caroline Blumer,{dagger}, and Dieter Haas*

Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland

Received 16 August 2001/ Accepted 19 November 2001

In Pseudomonas fluorescens CHA0, an antagonist of root-pathogenic fungi, the GacS/GacA two-component system tightly controls the expression of antifungal secondary metabolites and exoenzymes at a posttranscriptional level, involving the RNA-binding protein and global regulator of secondary metabolism RsmA. This protein was purified from P. fluorescens, and RNA bound to it was converted to cDNA, which served as a probe to isolate the corresponding chromosomal locus, rsmZ. This gene encoded a regulatory RNA of 127 nucleotides and a truncated form lacking 35 nucleotides at the 3" end. Expression of rsmZ depended on GacA, increased with increasing population density, and was stimulated by the addition of a solvent-extractable extracellular signal produced by strain CHA0 at the end of exponential growth. This signal appeared to be unrelated to N-acyl-homoserine lactones. A conserved upstream element in the rsmZ promoter, but not the stress sigma factor RpoS, was involved in rsmZ expression. Overexpression of rsmZ effectively suppressed the negative effect of gacS and gacA mutations on target genes, i.e., hcnA (for hydrogen cyanide synthase) and aprA (for the major exoprotease). Mutational inactivation of rsmZ resulted in reduced expression of these target genes in the presence of added signal. Overexpression of rsmA had a similar, albeit stronger negative effect. These results support a model in which GacA upregulates the expression of regulatory RNAs, such as RsmZ of strain CHA0, in response to a bacterial signal. By a titration effect, RsmZ may then alleviate the repressing activity of RsmA on the expression of target mRNAs.

* Corresponding author. Mailing address: Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 56 31. Fax: 41 21 692 56 35. E-mail: Dieter.Haas{at}lbm.unil.ch.

{dagger} Present address: Functional Genomics, Aventis Pharma Deutschland GmbH, D-65926 Frankfurt am Main, Germany.

Journal of Bacteriology, February 2002, p. 1046-1056, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1046-1056.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cody, W. L., Pritchett, C. L., Jones, A. K., Carterson, A. J., Jackson, D., Frisk, A., Wolfgang, M. C., Schurr, M. J. (2009). Pseudomonas aeruginosa AlgR Controls Cyanide Production in an AlgZ-Dependent Manner. J. Bacteriol. 191: 2993-3002 [Abstract] [Full Text]  
  • Sitthidet, C., Stevens, J. M., Chantratita, N., Currie, B. J., Peacock, S. J., Korbsrisate, S., Stevens, M. P. (2008). Prevalence and Sequence Diversity of a Factor Required for Actin-Based Motility in Natural Populations of Burkholderia Species. J. Clin. Microbiol. 46: 2418-2422 [Abstract] [Full Text]  
  • Fox, A., Haas, D., Reimmann, C., Heeb, S., Filloux, A., Voulhoux, R. (2008). Emergence of Secretion-Defective Sublines of Pseudomonas aeruginosa PAO1 Resulting from Spontaneous Mutations in the vfr Global Regulatory Gene. Appl. Environ. Microbiol. 74: 1902-1908 [Abstract] [Full Text]  
  • Krzeslak, J., Gerritse, G., van Merkerk, R., Cool, R. H., Quax, W. J. (2008). Lipase Expression in Pseudomonas alcaligenes Is Under the Control of a Two-Component Regulatory System. Appl. Environ. Microbiol. 74: 1402-1411 [Abstract] [Full Text]  
  • Zhang, X.-X., Rainey, P. B. (2008). Dual Involvement of CbrAB and NtrBC in the Regulation of Histidine Utilization in Pseudomonas fluorescens SBW25. Genetics 178: 185-195 [Abstract] [Full Text]  
  • Yang, S., Zhang, Q., Guo, J., Charkowski, A. O., Glick, B. R., Ibekwe, A. M., Cooksey, D. A., Yang, C.-H. (2007). Global Effect of Indole-3-Acetic Acid Biosynthesis on Multiple Virulence Factors of Erwinia chrysanthemi 3937. Appl. Environ. Microbiol. 73: 1079-1088 [Abstract] [Full Text]  
  • Yan, A., Huang, X., Liu, H., Dong, D., Zhang, D., Zhang, X., Xu, Y. (2007). An rhl-like quorum-sensing system negatively regulates pyoluteorin production in Pseudomonas sp. M18. Microbiology 153: 16-28 [Abstract] [Full Text]  
  • Dubuis, C., Haas, D. (2007). Cross-Species GacA-Controlled Induction of Antibiosis in Pseudomonads. Appl. Environ. Microbiol. 73: 650-654 [Abstract] [Full Text]  
  • Mondragon, V., Franco, B., Jonas, K., Suzuki, K., Romeo, T., Melefors, O., Georgellis, D. (2006). pH-Dependent Activation of the BarA-UvrY Two-Component System in Escherichia coli. J. Bacteriol. 188: 8303-8306 [Abstract] [Full Text]  
  • Girard, G., Barends, S., Rigali, S., van Rij, E. T., Lugtenberg, B. J. J., Bloemberg, G. V. (2006). Pip, a Novel Activator of Phenazine Biosynthesis in Pseudomonas chlororaphis PCL1391. J. Bacteriol. 188: 8283-8293 [Abstract] [Full Text]  
  • Kulkarni, P. R., Cui, X., Williams, J. W., Stevens, A. M., Kulkarni, R. V. (2006). Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri. Nucleic Acids Res 34: 3361-3369 [Abstract] [Full Text]  
  • Ryan, R. P., Fouhy, Y., Lucey, J. F., Crossman, L. C., Spiro, S., He, Y.-W., Zhang, L.-H., Heeb, S., Camara, M., Williams, P., Dow, J. M. (2006). Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover. Proc. Natl. Acad. Sci. USA 103: 6712-6717 [Abstract] [Full Text]  
  • Dubuis, C., Rolli, J., Lutz, M., Defago, G., Haas, D. (2006). Thiamine-Auxotrophic Mutants of Pseudomonas fluorescens CHA0 Are Defective in Cell-Cell Signaling and Biocontrol Factor Expression. Appl. Environ. Microbiol. 72: 2606-2613 [Abstract] [Full Text]  
  • Sio, C. F., Otten, L. G., Cool, R. H., Diggle, S. P., Braun, P. G., Bos, R., Daykin, M., Camara, M., Williams, P., Quax, W. J. (2006). Quorum Quenching by an N-Acyl-Homoserine Lactone Acylase from Pseudomonas aeruginosa PAO1. Infect. Immun. 74: 1673-1682 [Abstract] [Full Text]  
  • Ventre, I., Goodman, A. L., Vallet-Gely, I., Vasseur, P., Soscia, C., Molin, S., Bleves, S., Lazdunski, A., Lory, S., Filloux, A. (2006). Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes. Proc. Natl. Acad. Sci. USA 103: 171-176 [Abstract] [Full Text]  
  • Girard, G., van Rij, E. T., Lugtenberg, B. J. J., Bloemberg, G. V. (2006). Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391. Microbiology 152: 43-58 [Abstract] [Full Text]  
  • Zhao, Y., Blumer, S. E., Sundin, G. W. (2005). Identification of Erwinia amylovora Genes Induced during Infection of Immature Pear Tissue. J. Bacteriol. 187: 8088-8103 [Abstract] [Full Text]  
  • Kay, E., Dubuis, C., Haas, D. (2005). Three small RNAs jointly ensure secondary metabolism and biocontrol in Pseudomonas fluorescens CHA0. Proc. Natl. Acad. Sci. USA 102: 17136-17141 [Abstract] [Full Text]  
  • Stevens, J. M., Ulrich, R. L., Taylor, L. A., Wood, M. W., DeShazer, D., Stevens, M. P., Galyov, E. E. (2005). Actin-Binding Proteins from Burkholderia mallei and Burkholderia thailandensis Can Functionally Compensate for the Actin-Based Motility Defect of a Burkholderia pseudomallei bimA Mutant. J. Bacteriol. 187: 7857-7862 [Abstract] [Full Text]  
  • Siddiqui, I. A., Haas, D., Heeb, S. (2005). Extracellular Protease of Pseudomonas fluorescens CHA0, a Biocontrol Factor with Activity against the Root-Knot Nematode Meloidogyne incognita. Appl. Environ. Microbiol. 71: 5646-5649 [Abstract] [Full Text]  
  • Heurlier, K., Denervaud, V., Haenni, M., Guy, L., Krishnapillai, V., Haas, D. (2005). Quorum-Sensing-Negative (lasR) Mutants of Pseudomonas aeruginosa Avoid Cell Lysis and Death. J. Bacteriol. 187: 4875-4883 [Abstract] [Full Text]  
  • Brencic, A., Winans, S. C. (2005). Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria. Microbiol. Mol. Biol. Rev. 69: 155-194 [Abstract] [Full Text]  
  • Kobayashi, D. Y., Reedy, R. M., Palumbo, J. D., Zhou, J.-M., Yuen, G. Y. (2005). A clp Gene Homologue Belonging to the Crp Gene Family Globally Regulates Lytic Enzyme Production, Antimicrobial Activity, and Biological Control Activity Expressed by Lysobacter enzymogenes Strain C3. Appl. Environ. Microbiol. 71: 261-269 [Abstract] [Full Text]  
  • Reimmann, C., Valverde, C., Kay, E., Haas, D. (2005). Posttranscriptional Repression of GacS/GacA-Controlled Genes by the RNA-Binding Protein RsmE Acting Together with RsmA in the Biocontrol Strain Pseudomonas fluorescens CHA0. J. Bacteriol. 187: 276-285 [Abstract] [Full Text]  
  • Ovadis, M., Liu, X., Gavriel, S., Ismailov, Z., Chet, I., Chernin, L. (2004). The Global Regulator Genes from Biocontrol Strain Serratia plymuthica IC1270: Cloning, Sequencing, and Functional Studies. J. Bacteriol. 186: 4986-4993 [Abstract] [Full Text]  
  • Wilderman, P. J., Sowa, N. A., FitzGerald, D. J., FitzGerald, P. C., Gottesman, S., Ochsner, U. A., Vasil, M. L. (2004). Identification of tandem duplicate regulatory small RNAs in Pseudomonas aeruginosa involved in iron homeostasis. Proc. Natl. Acad. Sci. USA 101: 9792-9797 [Abstract] [Full Text]  
  • Valverde, C., Lindell, M., Wagner, E. G. H., Haas, D. (2004). A Repeated GGA Motif Is Critical for the Activity and Stability of the Riboregulator RsmY of Pseudomonas fluorescens. J. Biol. Chem. 279: 25066-25074 [Abstract] [Full Text]  
  • Bachman, M. A., Swanson, M. S. (2004). The LetE Protein Enhances Expression of Multiple LetA/LetS-Dependent Transmission Traits by Legionella pneumophila. Infect. Immun. 72: 3284-3293 [Abstract] [Full Text]  
  • Heurlier, K., Williams, F., Heeb, S., Dormond, C., Pessi, G., Singer, D., Camara, M., Williams, P., Haas, D. (2004). Positive Control of Swarming, Rhamnolipid Synthesis, and Lipase Production by the Posttranscriptional RsmA/RsmZ System in Pseudomonas aeruginosa PAO1. J. Bacteriol. 186: 2936-2945 [Abstract] [Full Text]  
  • Brodhagen, M., Henkels, M. D., Loper, J. E. (2004). Positive Autoregulation and Signaling Properties of Pyoluteorin, an Antibiotic Produced by the Biological Control Organism Pseudomonas fluorescens Pf-5. Appl. Environ. Microbiol. 70: 1758-1766 [Abstract] [Full Text]  
  • Whistler, C. A., Ruby, E. G. (2003). GacA Regulates Symbiotic Colonization Traits of Vibrio fischeri and Facilitates a Beneficial Association with an Animal Host. J. Bacteriol. 185: 7202-7212 [Abstract] [Full Text]  
  • Teplitski, M., Goodier, R. I., Ahmer, B. M. M. (2003). Pathways Leading from BarA/SirA to Motility and Virulence Gene Expression in Salmonella. J. Bacteriol. 185: 7257-7265 [Abstract] [Full Text]  
  • Whistler, C. A., Pierson III, L. S. (2003). Repression of Phenazine Antibiotic Production in Pseudomonas aureofaciens Strain 30-84 by RpeA. J. Bacteriol. 185: 3718-3725 [Abstract] [Full Text]  
  • Heurlier, K., Denervaud, V., Pessi, G., Reimmann, C., Haas, D. (2003). Negative Control of Quorum Sensing by RpoN ({sigma}54) in Pseudomonas aeruginosa PAO1. J. Bacteriol. 185: 2227-2235 [Abstract] [Full Text]  
  • Schmidt-Eisenlohr, H., Gast, A., Baron, C. (2003). Inactivation of gacS Does Not Affect the Competitiveness of Pseudomonas chlororaphis in the Arabidopsis thaliana Rhizosphere. Appl. Environ. Microbiol. 69: 1817-1826 [Abstract] [Full Text]  
  • Pernestig, A.-K., Georgellis, D., Romeo, T., Suzuki, K., Tomenius, H., Normark, S., Melefors, O. (2003). The Escherichia coli BarA-UvrY Two-Component System Is Needed for Efficient Switching between Glycolytic and Gluconeogenic Carbon Sources. J. Bacteriol. 185: 843-853 [Abstract] [Full Text]  
  • Suzuki, K., Wang, X., Weilbacher, T., Pernestig, A.-K., Melefors, O., Georgellis, D., Babitzke, P., Romeo, T. (2002). Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli. J. Bacteriol. 184: 5130-5140 [Abstract] [Full Text]  
  • Chatterjee, A., Cui, Y., Chatterjee, A. K. (2002). RsmA and the Quorum-Sensing Signal, N-[3-Oxohexanoyl]- L-Homoserine Lactone, Control the Levels of rsmB RNA in Erwinia carotovora subsp. carotovora by Affecting Its Stability. J. Bacteriol. 184: 4089-4095 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»