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Journal of Bacteriology, February 2002, p. 1102-1111, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1102-1111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the parB-Like yyaA Gene of Bacillus subtilis

Jörg Sievers,1,{dagger} Brian Raether,2 Marta Perego,2 and Jeff Errington1*

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom,1 Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 920372

Received 19 July 2001/ Accepted 13 November 2001

We have characterized the yyaA gene of Bacillus subtilis, located near the origin of chromosome replication (oriC). Its protein product is similar to the Spo0J protein, which belongs to the ParB family of chromosome- and plasmid-partitioning proteins. Insertional inactivation of the yyaA gene had no apparent effect on chromosome organization and partitioning during vegetative growth or sporulation. Subcellular localization of YyaA by immunofluorescence microscopy indicated that it colocalizes with the nucleoid, and gel retardation studies confirmed that YyaA binds relatively nonspecifically to DNA. Overexpression of yyaA caused a sporulation defect characterized by the formation of multiple septa within the cell. This phenotype indicates that YyaA may have a regulatory role at the onset of sporulation.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. Phone: (44) 1865 275561. Fax: (44) 1865 275556. E-mail: erring{at}molbiol.ox.ac.uk.

{dagger} Present address: Prolysis Ltd., Yarnton, Oxfordshire OX5 1PF, United Kingdom.

Journal of Bacteriology, February 2002, p. 1102-1111, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1102-1111.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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