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Journal of Bacteriology, February 2002, p. 1192-1195, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1192-1195.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Two "Wild-Type" Variants of Escherichia coli {sigma}70: Context-Dependent Effects of the Identity of Amino Acid 149

Nicole E. Baldwin, Andrea McCracken, and Alicia J. Dombroski*

Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas 77030

Received 17 August 2001/ Accepted 9 November 2001

The identity of amino acid 149 of Escherichia coli {sigma}70 has been reported variably as either arginine or aspartic acid. We show that the behavior of both a region 1.2 deletion and a single-amino-acid substitution at position 122 are greatly affected by the identity of amino acid 149.

* Corresponding author. Present address: NIH, Center for Scientific Review, 6701 Rockledge Dr., Mailstop 7808, Bethesda, MD 20892. Phone: (301) 435-1149. Fax: (301) 480-4042. E-mail: dombrosa{at}csr.nih.gov.

Journal of Bacteriology, February 2002, p. 1192-1195, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1192-1195.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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