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Journal of Bacteriology, February 2002, p. 1214-1218, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1214-1218.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces

Li-Fong Lee, Shun-Hua Yeh, and Carton W. Chen*

Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan

Received 23 August 2001/ Accepted 20 November 2001

Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to 30°C. This allowed manipulations to achieve replication synchronization.

* Corresponding author. Mailing address: Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan. Phone: 886-2-2826-7040. Fax: 886-2-2826-4930. E-mail: cwchen{at}ym.edu.tw.

Journal of Bacteriology, February 2002, p. 1214-1218, Vol. 184, No. 4
0021-9193/01/$04.00+0     DOI: 10.1128/jb.184.4.1214-1218.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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