This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Belaich, A. Articles by Belaich, J.-P. Search for Related Content PubMed PubMed Citation Articles by Belaich, A. Articles by Belaich, J.-P.

Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome

Laboratoire de Bioénergétique et Ingenierie des Protéines, IBSM,¹ Institut de Biochimie et Chimie des Protéines, Centre National de la Recherche Scientifique, Lyon,² Institut Méditerranééen de Recherche en Nutrition, Faculté des Sciences et Techniques de Saint Jérome,³ Université de Provence, Marseille, France⁴

Received 16 May 2001/ Accepted 25 November 2001

A new cellulosomal protein from Clostridium cellulolyticum Cel9M was characterized. The protein contains a catalytic domain belonging to family 9 and a dockerin domain. Cel9M is active on carboxymethyl cellulose, and the hydrolysis of this substrate is accompanied by a decrease in viscosity. Cel9M has a slight, albeit significant, activity on both Avicel and bacterial microcrystalline cellulose, and the main soluble sugar released is cellotetraose. Saccharification of bacterial microcrystalline cellulose by Cel9M in association with two other family 9 enzymes from C. cellulolyticum, namely, Cel9E and Cel9G, was measured, and it was found that Cel9M acts synergistically with Cel9E. Complexation of Cel9M with the mini-CipC1 containing the cellulose binding domain, the X2 domain, and the first cohesin domain of the scaffoldin CipC of the bacterium did not significantly increase the hydrolysis of Avicel and bacterial microcrystalline cellulose.

This article has been cited by other articles:

• Rincon, M. T., Cepeljnik, T., Martin, J. C., Barak, Y., Lamed, R., Bayer, E. A., Flint, H. J. (2007). A Novel Cell Surface-Anchored Cellulose-Binding Protein Encoded by the sca Gene Cluster of Ruminococcus flavefaciens. J. Bacteriol. 189: 4774-4783 [Abstract] [Full Text]  
• Mingardon, F., Chanal, A., Lopez-Contreras, A. M., Dray, C., Bayer, E. A., Fierobe, H.-P. (2007). Incorporation of Fungal Cellulases in Bacterial Minicellulosomes Yields Viable, Synergistically Acting Cellulolytic Complexes. Appl. Environ. Microbiol. 73: 3822-3832 [Abstract] [Full Text]  
• Koukiekolo, R., Cho, H.-Y., Kosugi, A., Inui, M., Yukawa, H., Doi, R. H. (2005). Degradation of Corn Fiber by Clostridium cellulovorans Cellulases and Hemicellulases and Contribution of Scaffolding Protein CbpA. Appl. Environ. Microbiol. 71: 3504-3511 [Abstract] [Full Text]  
• Fierobe, H.-P., Mingardon, F., Mechaly, A., Belaich, A., Rincon, M. T., Pages, S., Lamed, R., Tardif, C., Belaich, J.-P., Bayer, E. A. (2005). Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates: CONTROLLED INCORPORATION OF THREE DISTINCT ENZYMES INTO A DEFINED TRIFUNCTIONAL SCAFFOLDIN. J. Biol. Chem. 280: 16325-16334 [Abstract] [Full Text]  
• Perret, S., Belaich, A., Fierobe, H.-P., Belaich, J.-P., Tardif, C. (2004). Towards Designer Cellulosomes in Clostridia: Mannanase Enrichment of the Cellulosomes Produced by Clostridium cellulolyticum. J. Bacteriol. 186: 6544-6552 [Abstract] [Full Text]  
• Devillard, E., Goodheart, D. B., Karnati, S. K. R., Bayer, E. A., Lamed, R., Miron, J., Nelson, K. E., Morrison, M. (2004). Ruminococcus albus 8 Mutants Defective in Cellulose Degradation Are Deficient in Two Processive Endocellulases, Cel48A and Cel9B, Both of Which Possess a Novel Modular Architecture. J. Bacteriol. 186: 136-145 [Abstract] [Full Text]  
• Doi, R. H., Kosugi, A., Murashima, K., Tamaru, Y., Han, S. O. (2003). Cellulosomes from Mesophilic Bacteria. J. Bacteriol. 185: 5907-5914 [Full Text]  
• Pages, S., Valette, O., Abdou, L., Belaich, A., Belaich, J.-P. (2003). A Rhamnogalacturonan Lyase in the Clostridium cellulolyticum Cellulosome. J. Bacteriol. 185: 4727-4733 [Abstract] [Full Text]  
• Mandelman, D., Belaich, A., Belaich, J. P., Aghajari, N., Driguez, H., Haser, R. (2003). X-Ray Crystal Structure of the Multidomain Endoglucanase Cel9G from Clostridium cellulolyticum Complexed with Natural and Synthetic Cello-Oligosaccharides. J. Bacteriol. 185: 4127-4135 [Abstract] [Full Text]  
• Lopez-Contreras, A. M., Martens, A. A., Szijarto, N., Mooibroek, H., Claassen, P. A. M., van der Oost, J., de Vos, W. M. (2003). Production by Clostridium acetobutylicum ATCC 824 of CelG, a Cellulosomal Glycoside Hydrolase Belonging to Family 9. Appl. Environ. Microbiol. 69: 869-877 [Abstract] [Full Text]  
• Maamar, H., de Philip, P., Belaich, J.-P., Tardif, C. (2003). ISCce1 and ISCce2, Two Novel Insertion Sequences in Clostridium cellulolyticum. J. Bacteriol. 185: 714-725 [Abstract] [Full Text]  
• Arai, T., Araki, R., Tanaka, A., Karita, S., Kimura, T., Sakka, K., Ohmiya, K. (2003). Characterization of a Cellulase Containing a Family 30 Carbohydrate-Binding Module (CBM) Derived from Clostridium thermocellum CelJ: Importance of the CBM to Cellulose Hydrolysis. J. Bacteriol. 185: 504-512 [Abstract] [Full Text]