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Journal of Bacteriology, March 2002, p. 1444-1448, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1444-1448.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Charged Amino Acids Conserved in the Aromatic Acid/H+ Symporter Family of Permeases Are Required for 4-Hydroxybenzoate Transport by PcaK from Pseudomonas putida

*** Jayna L. Ditty,{dagger} and Caroline S. Harwood*

Department of Microbiology, The University of Iowa, Iowa City, Iowa 52242

Received 12 October 2001/ Accepted 6 December 2001

Charged amino acids in the predicted transmembrane portion of PcaK, a permease from Pseudomonas putida that transports 4-hydroxybenzoate (4-HBA), were required for 4-HBA transport, and they were also required for P. putida to have a chemotactic response to 4-HBA. An essential amino acid motif (DGXD) containing aspartate residues is located in the first transmembrane segment of PcaK and is conserved in the aromatic acid/H+ symporter family of the major facilitator superfamily of transporters.

* Corresponding author. Mailing address: Department of Microbiology, 3-432 BSB, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7783. Fax: (319) 335-7679. E-mail: caroline-harwood{at}uiowa.edu.

{dagger} Present address: Department of Biology, Texas A&M University, College Station, TX 77843.

Journal of Bacteriology, March 2002, p. 1444-1448, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1444-1448.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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