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Journal of Bacteriology, March 2002, p. 1498-1501, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1498-1501.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the petI and res Operons of Acidithiobacillus ferrooxidans

*** Gloria Levicán,1 Patrice Bruscella,2 Maritza Guacunano,1 Carolina Inostroza,1 Violaine Bonnefoy,2 David S. Holmes,1* and Eugenia Jedlicki3

Laboratory of Bioinformatics and Molecular Microbiology, University of Santiago,1 Institute for Biomedical Sciences, University of Chile, Santiago, Chile,3 Laboratory of Chemical Bacteriology, Institute of Structural Biology and Microbiology, CNRS, Marseille, France2

Received 16 May 2001/ Accepted 25 September 2001

DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-PCR experiments suggest that the two clusters are organized as operons, and +1 start sites of transcription for the operons have been determined by primer extension experiments. Potential promoters have been identified. The presence of these operons lends support to a recent model of reverse electron flow and is consistent with previous reports of phenotypic switching in this bacterium.

* Corresponding author. Mailing address: Department of Bioinformatics, University of Santiago (USACH), 3363 Alameda, Santiago Chile. Phone: 56-2-682-4749. Fax: 56-2-681-2108. E-mail: ejedlick{at}machi.med.uchile.cl.

Journal of Bacteriology, March 2002, p. 1498-1501, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1498-1501.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Bruscella, P., Appia-Ayme, C., Levican, G., Ratouchniak, J., Jedlicki, E., Holmes, D. S., Bonnefoy, V. (2007). Differential expression of two bc1 complexes in the strict acidophilic chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans suggests a model for their respective roles in iron or sulfur oxidation. Microbiology 153: 102-110 [Abstract] [Full Text]  
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