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Journal of Bacteriology, March 2002, p. 1498-1501, Vol. 184, No. 5
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.5.1498-1501.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of the petI and res Operons of Acidithiobacillus ferrooxidans

Laboratory of Bioinformatics and Molecular Microbiology, University of Santiago,¹ Institute for Biomedical Sciences, University of Chile, Santiago, Chile,³ Laboratory of Chemical Bacteriology, Institute of Structural Biology and Microbiology, CNRS, Marseille, France²

Received 16 May 2001/ Accepted 25 September 2001

DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-PCR experiments suggest that the two clusters are organized as operons, and +1 start sites of transcription for the operons have been determined by primer extension experiments. Potential promoters have been identified. The presence of these operons lends support to a recent model of reverse electron flow and is consistent with previous reports of phenotypic switching in this bacterium.

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