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Journal of Bacteriology, March 2002, p. 1794-1795, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1794-1795.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Lack of Regulation of the Modification-Dependent Restriction Enzyme McrBC in Escherichia coli

Mark Murphy, Stefanie Schmid Nuoffer, and Thomas A. Bickle*

Division of Molecular Microbiology, Biozentrum of the University of Basel, CH-4056 Basel, Switzerland

Received 19 October 2001/ Accepted 13 December 2001

Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.

* Corresponding author. Mailing address: Division of Molecular Microbiology, Biozentrum of the University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. Phone: 41 61 267 2120. Fax: 41 61 267 2118. E-mail: Thomas-A.Bickle{at}unibas.ch.

Journal of Bacteriology, March 2002, p. 1794-1795, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1794-1795.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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