This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drouin, F.
Right arrow Articles by Roy, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drouin, F.
Right arrow Articles by Roy, P. H.

 Previous Article

Journal of Bacteriology, March 2002, p. 1811-1815, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1811-1815.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The IntI-Like Tyrosine Recombinase of Shewanella oneidensis Is Active as an Integron Integrase

François Drouin,1 Josiane Mélançon,1,2 and Paul H. Roy1,2*

Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval,1 Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada2

Received 6 September 2001/ Accepted 10 December 2001

We have found an integron-like integrase gene and an attI site in Shewanella oneidensis as part of a small chromosomal integron. We have cloned this gene and tested the ability of the integrase to excise cassettes from various integrons. Most cassettes flanked by two attC sites are readily excised, while cassettes in the "first" position, with an attI1 or attI3 site on one end, are not excised. An exception is a cassette with attI2 on one end. The attI2 site, from Tn7, has greater similarity to the attI site adjacent to the integrase of S. oneidensis than do attI1 or attI3. We cloned the attI site of S. oneidensis and observed the integration of two different cassettes. We have, therefore, demonstrated the function of this integron-like integrase.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, CHUQ, Pavillon CHUL, Local RC-709, 2705 Boul. Laurier, Sainte-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Paul.H.Roy{at}crchul.ulaval.ca.

Journal of Bacteriology, March 2002, p. 1811-1815, Vol. 184, No. 6
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.6.1811-1815.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Larouche, A., Roy, P. H. (2009). Analysis by Mutagenesis of a Chromosomal Integron Integrase from Shewanella amazonensis SB2BT. J. Bacteriol. 191: 1933-1940 [Abstract] [Full Text]  
  • Tetu, S. G., Holmes, A. J. (2008). A Family of Insertion Sequences That Impacts Integrons by Specific Targeting of Gene Cassette Recombination Sites, the IS1111-attC Group. J. Bacteriol. 190: 4959-4970 [Abstract] [Full Text]  
  • Romine, M. F., Carlson, T. S., Norbeck, A. D., McCue, L. A., Lipton, M. S. (2008). Identification of Mobile Elements and Pseudogenes in the Shewanella oneidensis MR-1 Genome. Appl. Environ. Microbiol. 74: 3257-3265 [Abstract] [Full Text]  
  • Xu, H., Davies, J., Miao, V. (2007). Molecular Characterization of Class 3 Integrons from Delftia spp.. J. Bacteriol. 189: 6276-6283 [Abstract] [Full Text]  
  • Coleman, N. V., Holmes, A. J. (2005). The native Pseudomonas stutzeri strain Q chromosomal integron can capture and express cassette-associated genes. Microbiology 151: 1853-1864 [Abstract] [Full Text]  
  • Gillings, M. R., Holley, M. P., Stokes, H. W., Holmes, A. J. (2005). Integrons in Xanthomonas: A source of species genome diversity. Proc. Natl. Acad. Sci. USA 102: 4419-4424 [Abstract] [Full Text]  
  • Biskri, L., Bouvier, M., Guerout, A.-M., Boisnard, S., Mazel, D. (2005). Comparative Study of Class 1 Integron and Vibrio cholerae Superintegron Integrase Activities. J. Bacteriol. 187: 1740-1750 [Abstract] [Full Text]  
  • Collis, C. M., Hall, R. M. (2004). Comparison of the structure-activity relationships of the integron-associated recombination sites attI3 and attI1 reveals common features. Microbiology 150: 1591-1601 [Abstract] [Full Text]  
  • Hall, R. M., Stokes, H. W. (2004). Integrons or super integrons?. Microbiology 150: 3-4 [Full Text]  
  • Poirel, L., Heritier, C., Nordmann, P. (2004). Chromosome-Encoded Ambler Class D {beta}-Lactamase of Shewanella oneidensis as a Progenitor of Carbapenem-Hydrolyzing Oxacillinase. Antimicrob. Agents Chemother. 48: 348-351 [Abstract] [Full Text]  
  • Leon, G., Roy, P. H. (2003). Excision and Integration of Cassettes by an Integron Integrase of Nitrosomonas europaea. J. Bacteriol. 185: 2036-2041 [Abstract] [Full Text]  
  • Rowe-Magnus, D. A., Guerout, A.-M., Biskri, L., Bouige, P., Mazel, D. (2003). Comparative Analysis of Superintegrons: Engineering Extensive Genetic Diversity in the Vibrionaceae. Genome Res 13: 428-442 [Abstract] [Full Text]  
  • Holmes, A. J., Holley, M. P., Mahon, A., Nield, B., Gillings, M., Stokes, H. W. (2003). Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil. J. Bacteriol. 185: 918-928 [Abstract] [Full Text]  
  • Centron, D., Roy, P. H. (2002). Presence of a Group II Intron in a Multiresistant Serratia marcescens Strain That Harbors Three Integrons and a Novel Gene Fusion. Antimicrob. Agents Chemother. 46: 1402-1409 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»