This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tichi, M. A. Articles by Tabita, F. R. Search for Related Content PubMed PubMed Citation Articles by Tichi, M. A. Articles by Tabita, F. R.

Metabolic Signals That Lead to Control of CBB Gene Expression in Rhodobacter capsulatus

Department of Microbiology and Plant Molecular Biology/Biotechnology Program, The Ohio State University, Columbus, Ohio 43210-1292

Received 26 September 2001/ Accepted 9 January 2002

Various mutant strains were used to examine the regulation and metabolic control of the Calvin-Benson-Bassham (CBB) reductive pentose phosphate pathway in Rhodobacter capsulatus. Previously, a ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO)-deficient strain (strain SBI/II) was found to show enhanced levels of cbb_I and cbb_II promoter activities during photoheterotrophic growth in the presence of dimethyl sulfoxide. With this strain as the starting point, additional mutations were made in genes encoding phosphoribulokinase and transketolase and in the gene encoding the LysR-type transcriptional activator, CbbR_II. These strains revealed that a product generated by phosphoribulokinase was involved in control of CbbR-mediated cbb gene expression in SBI/II. Additionally, heterologous expression experiments indicated that Rhodobacter sphaeroides CbbR responded to the same metabolic signal in R. capsulatus SBI/II and mutant strain backgrounds.

This article has been cited by other articles:

• Uroz, S., Oger, P. M., Chapelle, E., Adeline, M.-T., Faure, D., Dessaux, Y. (2008). A Rhodococcus qsdA-Encoded Enzyme Defines a Novel Class of Large-Spectrum Quorum-Quenching Lactonases. Appl. Environ. Microbiol. 74: 1357-1366 [Abstract] [Full Text]  
• Toyoda, K., Yoshizawa, Y., Arai, H., Ishii, M., Igarashi, Y. (2005). The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110. Microbiology 151: 3615-3625 [Abstract] [Full Text]  
• Dubbs, P., Dubbs, J. M., Tabita, F. R. (2004). Effector-Mediated Interaction of CbbRI and CbbRII Regulators with Target Sequences in Rhodobacter capsulatus. J. Bacteriol. 186: 8026-8035 [Abstract] [Full Text]  
• Wei, X., Sayavedra-Soto, L. A., Arp, D. J. (2004). The transcription of the cbb operon in Nitrosomonas europaea. Microbiology 150: 1869-1879 [Abstract] [Full Text]  
• Kalyuzhnaya, M. G., Lidstrom, M. E. (2003). QscR, a LysR-Type Transcriptional Regulator and CbbR Homolog, Is Involved in Regulation of the Serine Cycle Genes in Methylobacterium extorquens AM1. J. Bacteriol. 185: 1229-1235 [Abstract] [Full Text]  
• Smith, S. A., Tabita, F. R. (2002). Up-Regulated Expression of the cbbI and cbbII Operons during Photoheterotrophic Growth of a Ribulose 1,5-Bisphosphate Carboxylase-Oxygenase Deletion Mutant of Rhodobacter sphaeroides. J. Bacteriol. 184: 6721-6724 [Abstract] [Full Text]