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Journal of Bacteriology, April 2002, p. 1947-1951, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1947-1951.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Chemiosmotic Energy Conservation with Na+ as the Coupling Ion during Hydrogen-Dependent Caffeate Reduction by Acetobacterium woodii

Frank Imkamp and Volker Müller*

Lehrstuhl für Mikrobiologie der Ludwig-Maximilians-Universität München, D-80638 Munich, Germany

Received 28 September 2001/ Accepted 9 January 2002

Cell suspensions of Acetobacterium woodii prepared from cultures grown on fructose plus caffeate catalyzed caffeate reduction with electrons derived from molecular hydrogen. Hydrogen-dependent caffeate reduction was strictly Na+ dependent with a Km for Na+ of 0.38 mM; Li+ could substitute for Na+. The sodium ionophore ETH2120, but not protonophores, stimulated hydrogen-dependent caffeate reduction by 280%, indicating that caffeate reduction is coupled to the buildup of a membrane potential generated by primary Na+ extrusion. Caffeate reduction was coupled to the synthesis of ATP, and again, ATP synthesis coupled to hydrogen-dependent caffeate reduction was strictly Na+ dependent and abolished by ETH2120, but not by protonophores, indicating the involvement of a transmembrane Na+ gradient in ATP synthesis. The ATPase inhibitor N,N'-dicyclohexylcarbodiimide (DCCD) abolished ATP synthesis, and at the same time, hydrogen-dependent caffeate reduction was inhibited. This inhibition could be relieved by ETH2120. These experiments are fully compatible with a chemiosmotic mechanism of ATP synthesis with Na+ as the coupling ion during hydrogen-dependent caffeate reduction by A. woodii.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie der Ludwig-Maximilians-Universität München, Maria-Ward-Str. 1a, D-80638 Munich, Germany. Phone: 49-89-21806126. Fax: 49-89-21806127. E-mail: v.mueller{at}lrz.uni-muenchen.de.

Journal of Bacteriology, April 2002, p. 1947-1951, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1947-1951.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Herrmann, G., Jayamani, E., Mai, G., Buckel, W. (2008). Energy Conservation via Electron-Transferring Flavoprotein in Anaerobic Bacteria. J. Bacteriol. 190: 784-791 [Full Text]  
  • Imkamp, F., Biegel, E., Jayamani, E., Buckel, W., Muller, V. (2007). Dissection of the Caffeate Respiratory Chain in the Acetogen Acetobacterium woodii: Identification of an Rnf-Type NADH Dehydrogenase as a Potential Coupling Site. J. Bacteriol. 189: 8145-8153 [Abstract] [Full Text]  
  • Dilling, S., Imkamp, F., Schmidt, S., Muller, V. (2007). Regulation of Caffeate Respiration in the Acetogenic Bacterium Acetobacterium woodii. Appl. Environ. Microbiol. 73: 3630-3636 [Abstract] [Full Text]  
  • Muller, V. (2003). Energy Conservation in Acetogenic Bacteria. Appl. Environ. Microbiol. 69: 6345-6353 [Full Text]  


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