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Journal of Bacteriology, April 2002, p. 1958-1965, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1958-1965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Physical Map of the Linear Chromosome of Streptomyces hygroscopicus 10-22 Deduced by Analysis of Overlapping Large Chromosomal Deletions

Xiuhua Pang,1,2 Xiufen Zhou,1,2 Yuhui Sun,1 and Zixin Deng1,2*

Bio-X Life Science Research Center, Shanghai Jiaotong University, Shanghai 200030,1 School of Life Science & Technology, Huazhong Agricultural University, Wuhan 430070, China2

Received 26 September 2001/ Accepted 11 January 2002

The chromosomal DNA of Streptomyces hygroscopicus 10-22, a derivative of strain 5102-6, was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestions with AseI gave 11 fragments with a total length of ca. 7.36 Mb. The AseI sites were mapped by analysis of overlapping chromosomal deletions in different mutants and confirmed by Southern hybridizations using partially digested genome fragments and linking cosmids as probes. PFGE analysis of DNA with and without proteinase K treatment, together with the hybridization results, suggested a linear organization with terminal proteins and large terminal inverted repeats. Some deletion mutants had circular chromosomes.

* Corresponding author. Mailing address: Bio-X Life Science Research Center, Shanghai Jiaotong University, Shanghai 200030, China. Phone: 86 21 62933404. Fax: 86 21 62933404. E-mail: zxdeng{at}mail.sjtu.edu.cn.

Journal of Bacteriology, April 2002, p. 1958-1965, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1958-1965.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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