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Journal of Bacteriology, April 2002, p. 1966-1973, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1966-1973.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Control of Butanol Formation in Clostridium acetobutylicum by Transcriptional Activation

Kai Thormann, Lothar Feustel, Karin Lorenz, Stephan Nakotte, and Peter Dürre*

Mikrobiologie und Biotechnologie, Universität Ulm, 89069 Ulm, Germany

Received 24 September 2001/ Accepted 4 January 2002

The sol operon of Clostridium acetobutylicum is the essential transcription unit for formation of the solvents butanol and acetone. The recent proposal that transcriptional regulation of this operon is controlled by the repressor Orf5/SolR (R. V. Nair, E. M. Green, D. E. Watson, G. N. Bennett, and E. T. Papoutsakis, J. Bacteriol. 181:319-330, 1999) was found to be incorrect. Instead, regulation depends on activation, most probably by the multivalent transcription factor Spo0A. The operon is transcribed from a single promoter. A second signal identified in primer extension studies results from mRNA processing and can be observed only in the natural host, not in a heterologous host. The first structural gene in the operon (adhE, encoding a bifunctional butyraldehyde/butanol dehydrogenase) is translated into two different proteins, the mature AdhE enzyme and the separate butanol dehydrogenase domain. The promoter of the sol operon is preceded by three imperfect repeats and a putative Spo0A-binding motif, which partially overlaps with repeat 3 (R3). Reporter gene analysis performed with the lacZ gene of Thermoanaerobacterium thermosulfurigenes and targeted mutations of the regulatory region revealed that the putative Spo0A-binding motif, R3, and R1 are essential for control. The data obtained also indicate that an additional activator protein is involved.

* Corresponding author. Mailing address: Mikrobiologie und Biotechnologie, Universität Ulm, 89069 Ulm, Germany. Phone: 49-731-50-22710. Fax: 49-731-50-22719. E-mail: peter.duerre{at}biologie.uni-ulm.de.

Journal of Bacteriology, April 2002, p. 1966-1973, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1966-1973.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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