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Journal of Bacteriology, April 2002, p. 1974-1987, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1974-1987.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Complete Genomic Sequence of SfV, a Serotype-Converting Temperate Bacteriophage of Shigella flexneri

Gwen E. Allison,,1 Dario Angeles,,2 Nai Tran-Dinh, and Naresh K. Verma*

School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra ACT 0200, Australia

Received 19 September 2001/ Accepted 8 January 2002

Bacteriophage SfV is a temperate serotype-converting phage of Shigella flexneri. SfV encodes the factors involved in type V O-antigen modification, and the serotype conversion and integration-excision modules of the phage have been isolated and characterized. We now report on the complete sequence of the SfV genome (37,074 bp). A total of 53 open reading frames were predicted from the nucleotide sequence, and analysis of the corresponding proteins was used to construct a functional map. The general organization of the genes in the SfV genome is similar to that of bacteriophage {lambda}, and numerous features of the sequence are described. The superinfection immunity system of SfV includes a lambda-like repression system and a P4-like transcription termination mechanism. Sequence analysis also suggests that SfV encodes multiple DNA methylases, and experiments confirmed that orf-41 encodes a Dam methylase. Studies conducted to determine if the phage-encoded methylase confers host DNA methylation showed that the two S. flexneri strains analyzed encode their own Dam methylase. Restriction mapping and sequence analysis revealed that the phage genome has cos sites at the termini. The tail assembly and structural genes of SfV show homology to those of phage Mu and Mu-like prophages in the genome of Escherichia coli O157:H7 and Haemophilus influenzae. Significant homology (30% of the genome in total) between sections of the early, regulatory, and structural regions of the SfV genome and the e14 and KpLE1 prophages in the E. coli K-12 genome were noted, suggesting that these three phages have common evolutionary origins.

* Corresponding author. Mailing address: Faculty of Science, School of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia. Phone: 61 2 6125 2666. Fax: 61 2 6125 0313. E-mail: Naresh.Verma{at}anu.edu.au.

{dagger} Present address: Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5.

{ddagger} Present address: Department of Microbiology, The National University of Singapore, Singapore 119074.

Journal of Bacteriology, April 2002, p. 1974-1987, Vol. 184, No. 7
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.7.1974-1987.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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