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Journal of Bacteriology, April 2002, p. 2081-2087, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2081-2087.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Anaerobic Respiration Using Fe3+, S0, and H2 in the Chemolithoautotrophic Bacterium Acidithiobacillus ferrooxidans

Naoya Ohmura,* Kazuhiro Sasaki, Norio Matsumoto, and Hiroshi Saiki

Department of Bio-Science, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-City Chiba 270-1194, Japan

Received 14 May 2001/ Accepted 7 January 2002

The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans has been known as an aerobe that respires on iron and sulfur. Here we show that the bacterium could chemolithoautotrophically grow not only on H2/O2 under aerobic conditions but also on H2/Fe3+, H2/S0, or S0/Fe3+ under anaerobic conditions. Anaerobic respiration using Fe3+ or S0 as an electron acceptor and H2 or S0 as an electron donor serves as a primary energy source of the bacterium. Anaerobic respiration based on reduction of Fe3+ induced the bacterium to synthesize significant amounts of a c-type cytochrome that was purified as an acid-stable and soluble 28-kDa monomer. The purified cytochrome in the oxidized form was reduced in the presence of the crude extract, and the reduced cytochrome was reoxidized by Fe3+. Respiration based on reduction of Fe3+ coupled to oxidation of a c-type cytochrome may be involved in the primary mechanism of energy production in the bacterium on anaerobic iron respiration.

* Corresponding author. Mailing address: Department of Bio-Science, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-City Chiba, Japan 270-1194. Phone: 81-471-82-1181. Fax: 81-471-83-3347. E-mail: ohmura{at}criepi.denken.or.jp.

Journal of Bacteriology, April 2002, p. 2081-2087, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2081-2087.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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