This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trieber, C. A.
Right arrow Articles by Taylor, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trieber, C. A.
Right arrow Articles by Taylor, D. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2002, p. 2131-2140, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2131-2140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations in the 16S rRNA Genes of Helicobacter pylori Mediate Resistance to Tetracycline

Catharine A. Trieber1 and Diane E. Taylor1,2*

Departments of Medical Microbiology and Immunology,1 Biological Sciences, University of Alberta, Edmonton, Alberta, Canada2

Received 21 November 2001/ Accepted 24 January 2002

Low-cost and rescue treatments for Helicobacter pylori infections involve combinations of several drugs including tetracycline. Resistance to tetracycline has recently emerged in H. pylori. The 16S rRNA gene sequences of two tetracycline-resistant clinical isolates (MIC = 64 µg/ml) were determined and compared to the consensus H. pylori 16S rRNA sequence. One isolate had four nucleotide substitutions, and the other had four substitutions and two deletions. Natural transformation with the 16S rRNA genes from the resistant organisms conferred tetracycline resistance on susceptible strains. 16S rRNA genes containing the individual mutations were constructed and tested for the ability to confer resistance. Only the 16S rRNA gene containing the triple mutation, AGA965-967TTC, was able to confer tetracycline resistance on H. pylori 26695. The MICs of tetracycline for the transformed strains were equivalent to those for the original clinical isolates. The two original isolates were also metronidazole resistant, but this trait was not linked to the tetracycline resistance phenotype. Serial passage of several H. pylori strains on increasing concentrations of tetracycline yielded mutants with only a very modest increase in tetracycline resistance to a MIC of 4 to 8 µg/ml. These mutants all had a deletion of G942 in the 16S rRNA genes. The mutations in the 16S rRNA are clearly responsible for tetracycline resistance in H. pylori.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 1-28 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-4777. Fax: (780) 492-7521. E-mail: diane.taylor{at}ualberta.ca.

Journal of Bacteriology, April 2002, p. 2131-2140, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2131-2140.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Barrow, K., Kwon, D. H. (2009). Alterations in Two-Component Regulatory Systems of phoPQ and pmrAB Are Associated with Polymyxin B Resistance in Clinical Isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 5150-5154 [Abstract] [Full Text]  
  • Anoushiravani, M., Falsafi, T., Niknam, V. (2009). Proton motive force-dependent efflux of tetracycline in clinical isolates of Helicobacter pylori. J Med Microbiol 58: 1309-1313 [Abstract] [Full Text]  
  • Degrange, S., Renaudin, H., Charron, A., Pereyre, S., Bebear, C., Bebear, C. M. (2008). Reduced susceptibility to tetracyclines is associated in vitro with the presence of 16S rRNA mutations in Mycoplasma hominis and Mycoplasma pneumoniae. J Antimicrob Chemother 61: 1390-1392 [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Glocker, E., Kist, M. (2006). Emergence of a Helicobacter pylori isolate with reduced susceptibility to tetracycline in Germany. J Antimicrob Chemother 58: 1103-1104 [Full Text]  
  • Binet, R., Maurelli, A. T. (2005). Fitness Cost Due to Mutations in the 16S rRNA Associated with Spectinomycin Resistance in Chlamydia psittaci 6BC. Antimicrob. Agents Chemother. 49: 4455-4464 [Abstract] [Full Text]  
  • Glocker, E., Berning, M., Gerrits, M. M., Kusters, J. G., Kist, M. (2005). Real-Time PCR Screening for 16S rRNA Mutations Associated with Resistance to Tetracycline in Helicobacter pylori. Antimicrob. Agents Chemother. 49: 3166-3170 [Abstract] [Full Text]  
  • Lawson, A. J., Elviss, N. C., Owen, R. J. (2005). Real-time PCR detection and frequency of 16S rDNA mutations associated with resistance and reduced susceptibility to tetracycline in Helicobacter pylori from England and Wales. J Antimicrob Chemother 56: 282-286 [Abstract] [Full Text]  
  • Nonaka, L., Connell, S. R., Taylor, D. E. (2005). 16S rRNA Mutations That Confer Tetracycline Resistance in Helicobacter pylori Decrease Drug Binding in Escherichia coli Ribosomes. J. Bacteriol. 187: 3708-3712 [Abstract] [Full Text]  
  • Xing, J. Z., Clarke, C., Zhu, L., Gabos, S. (2005). Development of a Microelectronic Chip Array for High-Throughput Genotyping of Helicobacter Species and Screening for Antimicrobial Resistance. J Biomol Screen 10: 235-245 [Abstract]  
  • Eardly, B. D., Nour, S. M., van Berkum, P., Selander, R. K. (2005). Rhizobial 16S rRNA and dnaK Genes: Mosaicism and the Uncertain Phylogenetic Placement of Rhizobium galegae. Appl. Environ. Microbiol. 71: 1328-1335 [Abstract] [Full Text]  
  • Wu, J. Y., Kim, J. J., Reddy, R., Wang, W. M., Graham, D. Y., Kwon, D. H. (2005). Tetracycline-Resistant Clinical Helicobacter pylori Isolates with and without Mutations in 16S rRNA-Encoding Genes. Antimicrob. Agents Chemother. 49: 578-583 [Abstract] [Full Text]  
  • Gerrits, M. M, van der Wouden, E.-J., Bax, D. A, van Zwet, A. A, van Vliet, A. H., de Jong, A., Kusters, J. G, Thijs, J. C, Kuipers, E. J (2004). Role of the rdxA and frxA genes in oxygen-dependent metronidazole resistance of Helicobacter pylori. J Med Microbiol 53: 1123-1128 [Abstract] [Full Text]  
  • Kasai, K., Kanno, T., Endo, Y., Wakasa, K., Tozawa, Y. (2004). Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline. Nucleic Acids Res 32: 5732-5741 [Abstract] [Full Text]  
  • Gibreel, A., Tracz, D. M., Nonaka, L., Ngo, T. M., Connell, S. R., Taylor, D. E. (2004). Incidence of Antibiotic Resistance in Campylobacter jejuni Isolated in Alberta, Canada, from 1999 to 2002, with Special Reference to tet(O)-Mediated Tetracycline Resistance. Antimicrob. Agents Chemother. 48: 3442-3450 [Abstract] [Full Text]  
  • Megraud, F (2004). H pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut 53: 1374-1384 [Full Text]  
  • Anokhina, M. M., Barta, A., Nierhaus, K. H., Spiridonova, V. A., Kopylov, A. M. (2004). Mapping of the second tetracycline binding site on the ribosomal small subunit of E.coli. Nucleic Acids Res 32: 2594-2597 [Abstract] [Full Text]  
  • Bauer, G., Berens, C., Projan, S. J., Hillen, W. (2004). Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA. J Antimicrob Chemother 53: 592-599 [Abstract] [Full Text]  
  • Connell, S. R., Tracz, D. M., Nierhaus, K. H., Taylor, D. E. (2003). Ribosomal Protection Proteins and Their Mechanism of Tetracycline Resistance. Antimicrob. Agents Chemother. 47: 3675-3681 [Full Text]  
  • Gerrits, M. M., Berning, M., Van Vliet, A. H. M., Kuipers, E. J., Kusters, J. G. (2003). Effects of 16S rRNA Gene Mutations on Tetracycline Resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 47: 2984-2986 [Abstract] [Full Text]  
  • Kwon, D. H., Dore, M. P., Kim, J. J., Kato, M., Lee, M., Wu, J. Y., Graham, D. Y. (2003). High-Level {beta}-Lactam Resistance Associated with Acquired Multidrug Resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 47: 2169-2178 [Abstract] [Full Text]  
  • Ribera, A., Ruiz, J., Vila, J. (2003). Presence of the Tet M Determinant in a Clinical Isolate of Acinetobacter baumannii. Antimicrob. Agents Chemother. 47: 2310-2312 [Abstract] [Full Text]  
  • Kersulyte, D., Velapatino, B., Mukhopadhyay, A. K., Cahuayme, L., Bussalleu, A., Combe, J., Gilman, R. H., Berg, D. E. (2003). Cluster of Type IV Secretion Genes in Helicobacter pylori's Plasticity Zone. J. Bacteriol. 185: 3764-3772 [Abstract] [Full Text]  
  • Dailidiene, D., Bertoli, M. T., Miciuleviciene, J., Mukhopadhyay, A. K., Dailide, G., Pascasio, M. A., Kupcinskas, L., Berg, D. E. (2002). Emergence of Tetracycline Resistance in Helicobacter pylori: Multiple Mutational Changes in 16S Ribosomal DNA and Other Genetic Loci. Antimicrob. Agents Chemother. 46: 3940-3946 [Abstract] [Full Text]  
  • Gerrits, M. M., de Zoete, M. R., Arents, N. L. A., Kuipers, E. J., Kusters, J. G. (2002). 16S rRNA Mutation-Mediated Tetracycline Resistance in Helicobacter pylori. Antimicrob. Agents Chemother. 46: 2996-3000 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»