This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kojic, M.
Right arrow Articles by Venturi, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kojic, M.
Right arrow Articles by Venturi, V.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2002, p. 2324-2330, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2324-2330.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

TetR Family Member PsrA Directly Binds the Pseudomonas rpoS and psrA Promoters

Milan Kojic, Claudio Aguilar, and Vittorio Venturi*

Bacteriology Group, International Centre for Genetic Engineering & Biotechnology, 34012 Trieste, Italy

Received 27 September 2001/ Accepted 8 January 2002

We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions -59 to -35 in the rpoS promoter and from -18 to +20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.

* Corresponding author. Mailing address: Bacteriology Group, International Centre for Genetic Engineering & Biotechnology, Area Science Park, Padriciano 99, 34012 Trieste, Italy. Phone: 040 3757317. Fax: 040 226555. E-mail: venturi{at}icgeb.trieste.it.

Journal of Bacteriology, April 2002, p. 2324-2330, Vol. 184, No. 8
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.8.2324-2330.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kazakov, A. E., Rodionov, D. A., Alm, E., Arkin, A. P., Dubchak, I., Gelfand, M. S. (2009). Comparative Genomics of Regulation of Fatty Acid and Branched-Chain Amino Acid Utilization in Proteobacteria. J. Bacteriol. 191: 52-64 [Abstract] [Full Text]  
  • Lee, H.-J., Park, S.-J., Choi, S. H., Lee, K.-H. (2008). Vibrio vulnificus rpoS Expression Is Repressed by Direct Binding of cAMP-cAMP Receptor Protein Complex to Its Two Promoter Regions. J. Biol. Chem. 283: 30438-30450 [Abstract] [Full Text]  
  • Gooderham, W. J., Bains, M., McPhee, J. B., Wiegand, I., Hancock, R. E. W. (2008). Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa. J. Bacteriol. 190: 5624-5634 [Abstract] [Full Text]  
  • Kang, Y., Nguyen, D. T., Son, M. S., Hoang, T. T. (2008). The Pseudomonas aeruginosa PsrA responds to long-chain fatty acid signals to regulate the fadBA5 {beta}-oxidation operon. Microbiology 154: 1584-1598 [Abstract] [Full Text]  
  • Chatterjee, A., Cui, Y., Hasegawa, H., Chatterjee, A. K. (2007). PsrA, the Pseudomonas Sigma Regulator, Controls Regulators of Epiphytic Fitness, Quorum-Sensing Signals, and Plant Interactions in Pseudomonas syringae pv. tomato Strain DC3000. Appl. Environ. Microbiol. 73: 3684-3694 [Abstract] [Full Text]  
  • Shen, D. K., Filopon, D., Kuhn, L., Polack, B., Toussaint, B. (2006). PsrA Is a Positive Transcriptional Regulator of the Type III Secretion System in Pseudomonas aeruginosa. Infect. Immun. 74: 1121-1129 [Abstract] [Full Text]  
  • Girard, G., van Rij, E. T., Lugtenberg, B. J. J., Bloemberg, G. V. (2006). Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391. Microbiology 152: 43-58 [Abstract] [Full Text]  
  • Preiter, K., Brooks, D. M., Penaloza-Vazquez, A., Sreedharan, A., Bender, C. L., Kunkel, B. N. (2005). Novel Virulence Gene of Pseudomonas syringae pv. tomato Strain DC3000. J. Bacteriol. 187: 7805-7814 [Abstract] [Full Text]  
  • Bertani, I., Venturi, V. (2004). Regulation of the N-Acyl Homoserine Lactone-Dependent Quorum-Sensing System in Rhizosphere Pseudomonas putida WCS358 and Cross-Talk with the Stationary-Phase RpoS Sigma Factor and the Global Regulator GacA. Appl. Environ. Microbiol. 70: 5493-5502 [Abstract] [Full Text]  
  • Sevo, M., Buratti, E., Venturi, V. (2004). Ribosomal Protein S1 Specifically Binds to the 5' Untranslated Region of the Pseudomonas aeruginosa Stationary-Phase Sigma Factor rpoS mRNA in the Logarithmic Phase of Growth. J. Bacteriol. 186: 4903-4909 [Abstract] [Full Text]  
  • Subsin, B., Thomas, M. S., Katzenmeier, G., Shaw, J. G., Tungpradabkul, S., Kunakorn, M. (2003). Role of the Stationary Growth Phase Sigma Factor RpoS of Burkholderia pseudomallei in Response to Physiological Stress Conditions. J. Bacteriol. 185: 7008-7014 [Abstract] [Full Text]  
  • Rojas, A., Segura, A., Guazzaroni, M. E., Teran, W., Hurtado, A., Gallegos, M. T., Ramos, J. L. (2003). In Vivo and In Vitro Evidence that TtgV Is the Specific Regulator of the TtgGHI Multidrug and Solvent Efflux Pump of Pseudomonas putida. J. Bacteriol. 185: 4755-4763 [Abstract] [Full Text]  
  • Parschat, K., Hauer, B., Kappl, R., Kraft, R., Huttermann, J., Fetzner, S. (2003). Gene Cluster of Arthrobacter ilicis Ru61a Involved in the Degradation of Quinaldine to Anthranilate: CHARACTERIZATION AND FUNCTIONAL EXPRESSION OF THE QUINALDINE 4-OXIDASE qoxLMS GENES. J. Biol. Chem. 278: 27483-27494 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»