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Journal of Bacteriology, May 2002, p. 2344-2351, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2344-2351.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK

School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka 424-8610,¹ Faculty of Engineering, Fukuyama University, Fukuyama 729-0292,² Nara Advanced Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-01, Japan³

Received 5 November 2001/ Accepted 26 January 2002

The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known previously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.

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