This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hale, C. A. Articles by de Boer, P. A. J. Search for Related Content PubMed PubMed Citation Articles by Hale, C. A. Articles by de Boer, P. A. J.

ZipA Is Required for Recruitment of FtsK, FtsQ, FtsL, and FtsN to the Septal Ring in Escherichia coli

Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960

Received 11 December 2001/ Accepted 1 February 2002

The septal ring in Escherichia coli consists of at least nine essential gene products whose order of assembly resembles a mostly linear dependency pathway: FtsA and ZipA directly bind FtsZ polymers at the prospective division site, followed by the sequential addition of FtsK, FtsQ, FtsL, FtsW, FtsI, and FtsN. Recruitment of FtsK and all downstream components requires the prior localization of FtsA. Here we show that recruitment of FtsK, FtsQ, FtsL, and FtsN equally requires ZipA. The results imply that association of both FtsA and ZipA with FtsZ polymers is needed for further maturation of the nascent organelle.

This article has been cited by other articles:

• Li, J., Attila, C., Wang, L., Wood, T. K., Valdes, J. J., Bentley, W. E. (2007). Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture. J. Bacteriol. 189: 6011-6020 [Abstract] [Full Text]  
• Corbin, B. D., Wang, Y., Beuria, T. K., Margolin, W. (2007). Interaction between Cell Division Proteins FtsE and FtsZ. J. Bacteriol. 189: 3026-3035 [Abstract] [Full Text]  
• Ramos, A., Letek, M., Campelo, A. B., Vaquera, J., Mateos, L. M., Gil, J. A. (2005). Altered morphology produced by ftsZ expression in Corynebacterium glutamicum ATCC 13869. Microbiology 151: 2563-2572 [Abstract] [Full Text]  
• Karimova, G., Dautin, N., Ladant, D. (2005). Interaction Network among Escherichia coli Membrane Proteins Involved in Cell Division as Revealed by Bacterial Two-Hybrid Analysis. J. Bacteriol. 187: 2233-2243 [Abstract] [Full Text]  
• Addinall, S. G., Small, E., Whitaker, D., Sturrock, S., Donachie, W. D., Khattar, M. M. (2005). New Temperature-Sensitive Alleles of ftsZ in Escherichia coli. J. Bacteriol. 187: 358-365 [Abstract] [Full Text]  
• Goehring, N. W., Gueiros-Filho, F., Beckwith, J. (2005). Premature targeting of a cell division protein to midcell allows dissection of divisome assembly in Escherichia coli. Genes Dev. 19: 127-137 [Abstract] [Full Text]  
• Marrington, R., Small, E., Rodger, A., Dafforn, T. R., Addinall, S. G. (2004). FtsZ Fiber Bundling Is Triggered by a Conformational Change in Bound GTP. J. Biol. Chem. 279: 48821-48829 [Abstract] [Full Text]  
• Corbin, B. D., Geissler, B., Sadasivam, M., Margolin, W. (2004). Z-Ring-Independent Interaction between a Subdomain of FtsA and Late Septation Proteins as Revealed by a Polar Recruitment Assay. J. Bacteriol. 186: 7736-7744 [Abstract] [Full Text]  
• Ferguson, P. L., Shaw, G. S. (2004). Human S100B Protein Interacts with the Escherichia coli Division Protein FtsZ in a Calcium-sensitive Manner. J. Biol. Chem. 279: 18806-18813 [Abstract] [Full Text]  
• Johnson, J. E., Lackner, L. L., Hale, C. A., de Boer, P. A. J. (2004). ZipA Is Required for Targeting of DMinC/DicB, but Not DMinC/MinD, Complexes to Septal Ring Assemblies in Escherichia coli. J. Bacteriol. 186: 2418-2429 [Abstract] [Full Text]  
• Schmidt, K. L., Peterson, N. D., Kustusch, R. J., Wissel, M. C., Graham, B., Phillips, G. J., Weiss, D. S. (2004). A Predicted ABC Transporter, FtsEX, Is Needed for Cell Division in Escherichia coli. J. Bacteriol. 186: 785-793 [Abstract] [Full Text]  
• Di Lallo, G., Fagioli, M., Barionovi, D., Ghelardini, P., Paolozzi, L. (2003). Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation. Microbiology 149: 3353-3359 [Abstract] [Full Text]  
• Vitha, S., Froehlich, J. E., Koksharova, O., Pyke, K. A., van Erp, H., Osteryoung, K. W. (2003). ARC6 Is a J-Domain Plastid Division Protein and an Evolutionary Descendant of the Cyanobacterial Cell Division Protein Ftn2. Plant Cell 15: 1918-1933 [Abstract] [Full Text]  
• Eberhardt, C., Kuerschner, L., Weiss, D. S. (2003). Probing the Catalytic Activity of a Cell Division-Specific Transpeptidase In Vivo with {beta}-Lactams. J. Bacteriol. 185: 3726-3734 [Abstract] [Full Text]  
• Du, Y., Arvidson, C. G. (2003). Identification of ZipA, a Signal Recognition Particle-Dependent Protein from Neisseria gonorrhoeae. J. Bacteriol. 185: 2122-2130 [Abstract] [Full Text]  
• Geissler, B., Elraheb, D., Margolin, W. (2003). A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli. Proc. Natl. Acad. Sci. USA 100: 4197-4202 [Abstract] [Full Text]  
• Errington, J., Daniel, R. A., Scheffers, D.-J. (2003). Cytokinesis in Bacteria. Microbiol. Mol. Biol. Rev. 67: 52-65 [Abstract] [Full Text]  
• Lackner, L. L., Raskin, D. M., de Boer, P. A. J. (2003). ATP-Dependent Interactions between Escherichia coli Min Proteins and the Phospholipid Membrane In Vitro. J. Bacteriol. 185: 735-749 [Abstract] [Full Text]  
• Hamoen, L. W., Errington, J. (2003). Polar Targeting of DivIVA in Bacillus subtilis Is Not Directly Dependent on FtsZ or PBP 2B. J. Bacteriol. 185: 693-697 [Abstract] [Full Text]