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Journal of Bacteriology, May 2002, p. 2561-2566, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2561-2566.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Plasmid pRT1 from Pyrococcus sp. Strain JT1

Donald E. Ward,^1* Ingrid M. Revet,¹ Renu Nandakumar,² Jon H. Tuttle,³ Willem M. de Vos,¹ John van der Oost,¹ and Jocelyne DiRuggiero²

Laboratory for Microbiology, Wageningen University, 6703CT Wageningen, The Netherlands,¹ Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742,² Chesapeake Biological Laboratory, Center for Environmental and Estuarine Studies, University of Maryland System, Solomons, Maryland 20688³

Received 30 August 2001/ Accepted 7 February 2002

We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.

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* Corresponding author. Present address: Department of Chemical Engineering, North Carolina State University, Campus Box 7270, Raleigh, NC 27695-7270. Phone: (919) 515-4452. Fax: (919) 515-3465. E-mail: deward{at}unity.ncsu.edu.

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Journal of Bacteriology, May 2002, p. 2561-2566, Vol. 184, No. 9
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.9.2561-2566.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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