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Journal of Bacteriology, January 2003, p. 155-164, Vol. 185, No. 1
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.1.155-164.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcriptome Analysis of Neisseria meningitidis during Infection

Guido Dietrich,1,{dagger} Sebastian Kurz,1 Claudia Hübner,1 Christian Aepinus,1 Stephanie Theiss,1 Matthias Guckenberger,1 Ursula Panzner,1 Jacqueline Weber,2 and Matthias Frosch1*

Institute for Hygiene and Microbiology, University of Würzburg, 97080 Würzburg,1 MWG-Biotech AG, 85560 Ebersberg, Germany2

Received 15 August 2002/ Accepted 2 October 2002

Neisseria meningitidis is the cause of septicemia and meningococcal meningitis. During the course of infection, N. meningitidis encounters multiple environments within its host, which makes rapid adaptation to environmental changes a crucial factor for neisserial pathogenicity. Employing oligonucleotide-based DNA microarrays, we analyzed the transcriptome of N. meningitidis during two key steps of meningococcal infection, i.e., the interaction with epithelial cells (HeLa cells) and endothelial cells (human brain microvascular endothelial cells). Seventy-two genes were differentially regulated after contact with epithelial cells, and 48 genes were differentially regulated after contact with endothelial cells, including a considerable proportion of well-known virulence genes. While a considerable number of genes were in concordance between bacteria adherent to both cell types, we identified several open reading frames that were differentially regulated in only one system. The data obtained with this novel approach may provide insight into the pathogenicity mechanisms of N. meningitidis and could demonstrate the importance of gene regulation on the transcriptional level during different stages of meningococcal infection.

* Corresponding author. Mailing address: Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany. Phone: 49-931-201-46160. Fax: 49-931-201-46445. E-mail: mfrosch{at}hygiene.uni-wuerzburg.de.

{dagger} Present address: Berna Biotech Ltd., Bacterial Vaccine Research, 3018 Berne, Switzerland.

Journal of Bacteriology, January 2003, p. 155-164, Vol. 185, No. 1
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.1.155-164.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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