This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alexeeva, S. Articles by Teixeira de Mattos, M. J. Search for Related Content PubMed PubMed Citation Articles by Alexeeva, S. Articles by Teixeira de Mattos, M. J.

Requirement of ArcA for Redox Regulation in Escherichia coli under Microaerobic but Not Anaerobic or Aerobic Conditions

Svetlana Alexeeva, Klaas J. Hellingwerf, and M. Joost Teixeira de Mattos^*

Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1018WV Amsterdam, The Netherlands

Received 13 June 2002/ Accepted 2 October 2002

In Escherichia coli, the two-component regulatory ArcAB system functions as a major control system for the regulation of expression of genes encoding enzymes involved in both aerobic and anaerobic catabolic pathways. Previously, we have described the physiological response of wild-type E. coli to changes in oxygen availability through the complete range from anaerobiosis to full aerobiosis (S. Alexeeva, B. de Kort, G. Sawers, K. J. Hellingwerf, and M. J. Teixeira de Mattos, J. Bacteriol. 182:4934-4940, 2000, and S. Alexeeva, K. J. Hellingwerf, and M. J. Teixeira de Mattos, J. Bacteriol. 184:1402-1406, 2002). Here, we address the question of the contribution of the ArcAB-dependent transcriptional regulation to this response. Wild-type E. coli and a mutant lacking the ArcA regulator were grown in glucose-limited chemostat cultures at controlled levels of oxygen availability ranging from full aerobiosis to complete anaerobiosis. A flux analysis of the distribution of catabolic fluxes over parallel pathways was carried out, and the intracellular redox state (as reflected by the NADH/NAD ratio) was monitored for all steady states. Deletion of ArcA neither significantly altered the in vivo activity of the pyruvate dehydrogenase complex and pyruvate formate lyase nor significantly affected catabolism under fully aerobic and fully anaerobic conditions. In contrast, profound effects of the absence of ArcA were seen under conditions of oxygen-restricted growth: increased respiration, an altered electron flux distribution over the cytochrome o- and d-terminal oxidases, and a significant change in the intracellular redox state were observed. Thus, the ArcA regulator was found to exert major control on flux distribution, and it is concluded that the ArcAB system should be considered a microaerobic redox regulator.

Present address: Dept. of Ophthalmogenetics, Ophthalmic Research Institute, 1105 BA Amsterdam, The Netherlands.

This article has been cited by other articles:

• Nikel, P. I., de Almeida, A., Pettinari, M. J., Mendez, B. S. (2008). The Legacy of HfrH: Mutations in the Two-Component System CreBC Are Responsible for the Unusual Phenotype of an Escherichia coli arcA Mutant. J. Bacteriol. 190: 3404-3407 [Abstract] [Full Text]  
• Beek, A. T., Keijser, B. J. F., Boorsma, A., Zakrzewska, A., Orij, R., Smits, G. J., Brul, S. (2008). Transcriptome Analysis of Sorbic Acid-Stressed Bacillus subtilis Reveals a Nutrient Limitation Response and Indicates Plasma Membrane Remodeling. J. Bacteriol. 190: 1751-1761 [Abstract] [Full Text]  
• Jones, S. A., Chowdhury, F. Z., Fabich, A. J., Anderson, A., Schreiner, D. M., House, A. L., Autieri, S. M., Leatham, M. P., Lins, J. J., Jorgensen, M., Cohen, P. S., Conway, T. (2007). Respiration of Escherichia coli in the Mouse Intestine. Infect. Immun. 75: 4891-4899 [Abstract] [Full Text]  
• Nikel, P. I., Pettinari, M. J., Galvagno, M. A., Mendez, B. S. (2006). Poly(3-Hydroxybutyrate) Synthesis by Recombinant Escherichia coli arcA Mutants in Microaerobiosis. Appl. Environ. Microbiol. 72: 2614-2620 [Abstract] [Full Text]  
• Bresolin, G., Neuhaus, K., Scherer, S., Fuchs, T. M. (2006). Transcriptional Analysis of Long-Term Adaptation of Yersinia enterocolitica to Low-Temperature Growth.. J. Bacteriol. 188: 2945-2958 [Abstract] [Full Text]  
• Mika, F., Hengge, R. (2005). A two-component phosphotransfer network involving ArcB, ArcA, and RssB coordinates synthesis and proteolysis of {sigma}S (RpoS) in E. coli. Genes Dev. 19: 2770-2781 [Abstract] [Full Text]  
• Yoshida, A., Nishimura, T., Kawaguchi, H., Inui, M., Yukawa, H. (2005). Enhanced Hydrogen Production from Formic Acid by Formate Hydrogen Lyase-Overexpressing Escherichia coli Strains. Appl. Environ. Microbiol. 71: 6762-6768 [Abstract] [Full Text]  
• BAILEY-SERRES, J., CHANG, R. (2005). Sensing and Signalling in Response to Oxygen Deprivation in Plants and Other Organisms. ANN BOT (LOND) 96: 507-518 [Abstract] [Full Text]  
• Perrenoud, A., Sauer, U. (2005). Impact of Global Transcriptional Regulation by ArcA, ArcB, Cra, Crp, Cya, Fnr, and Mlc on Glucose Catabolism in Escherichia coli. J. Bacteriol. 187: 3171-3179 [Abstract] [Full Text]