Journal of Bacteriology, January 2003, p. 377-380, Vol. 185, No. 1
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.1.377-380.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
The Pseudomonas aeruginosa rhlAB Operon Is Not Expressed during the Logarithmic Phase of Growth Even in the Presence of Its Activator RhlR and the Autoinducer N-Butyryl-Homoserine Lactone
Gerardo Medina, Katy Juárez, and Gloria Soberón-Chávez*
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Received 19 July 2002/
Accepted 14 October 2002
The Pseudomonas aeruginosa rhlAB operon encodes the enzyme rhamnosyltransferase 1, which produces the biosurfactant mono-rhamnolipid; rhlAB induction is dependent on the quorum-sensing transcription activator RhlR complexed with the autoinducer N-butyryl-homoserine lactone (C4-HSL). In this work we studied rhlAB induction in a P. aeruginosa and Escherichia coli background. We found that, in both bacteria, its expression is not induced during the logarithmic phase of growth even in the presence of RhlR and C4-HSL. Additionally, we found that rhlAB expression is partially
s dependent.
* Corresponding author. Mailing address: Departamento de Microbiología Molecular, Instituto de Biotecnología, UNAM, Apdo. Postal 510-3, Cuernavaca Morelos 62251, Mexico. Phone: (52) (777) 3291634. Fax: (52) (777) 3172388. E-mail: gloria{at}ibt.unam.mx.
Journal of Bacteriology, January 2003, p. 377-380, Vol. 185, No. 1
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.1.377-380.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
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Copyright © 2003 by the American Society for Microbiology. All rights reserved.