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Journal of Bacteriology, May 2003, p. 3179-3189, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3179-3189.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Global Stage-Specific Gene Regulation during the Developmental Cycle of Chlamydia trachomatis

Tracy L. Nicholson,1 Lynn Olinger,2 Kimberley Chong,3 Gary Schoolnik,3 and Richard S. Stephens1,2*

Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720,1 Francis I. Proctor Foundation, University of California, San Francisco, California 94143,2 Department of Microbiology and Immunology, Stanford University Medical Center, Stanford, California 943053

Received 18 December 2002/ Accepted 26 February 2003

Distinct morphological changes associated with the complex development cycle of the obligate intracellular bacterial pathogen Chlamydia trachomatis have been historically well characterized by microscopy. A number of temporally regulated genes have been characterized previously, suggesting that the chlamydial developmental cycle is regulated at the transcriptional level. This hypothesis was tested by microarray analysis in which the entire C. trachomatis genome was analyzed, providing a comprehensive assessment of global gene regulation throughout the chlamydial developmental cycle. Seven temporally cohesive gene clusters were identified, with 22% (189 genes) of the genome differentially expressed during the developmental cycle. The correlation of these gene clusters with hallmark morphological events of the chlamydial developmental cycle suggests three global stage-specific networks of gene regulation.

* Corresponding author. Mailing address: Division of Infectious Diseases, School of Public Health, 140 Earl Warren Hall, University of California, Berkeley, CA 94720-7360. Phone: (510) 643-9900. Fax: (510) 643-1537. E-mail: rss{at}uclink4.berkeley.edu.

Journal of Bacteriology, May 2003, p. 3179-3189, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3179-3189.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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