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Journal of Bacteriology, May 2003, p. 3210-3213, Vol. 185, No. 10
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.10.3210-3213.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Laboratory of Microbiology, Wageningen University, 6703 CT Wageningen,1 Kluyver Department of Biotechnology, University of Delft, 2628 BC Delft, The Netherlands,3 Institute for Organic Chemistry and Biochemistry, University of Freiburg, D-79104 Freiburg, Germany2
Received 23 December 2002/ Accepted 26 February 2003
A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also found in other dissimilatory oxyanion reductases.
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