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The yggH Gene of Escherichia coli Encodes a tRNA (m⁷G46) Methyltransferase

Laboratoire de Microbiologie, Université Libre de Bruxelles,¹ Institut de Recherches Microbiologiques Jean-Marie Wiame, B-1070 Brussels,² Laboratoire de Chimie Biologique, Université de Mons-Hainaut, B-7000 Mons, Belgium,³ Bioinformatics Laboratory, International Institute of Molecular and Cell Biology, 02-109 Warsaw, Poland⁴

Received 20 September 2002/ Accepted 3 March 2003

We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N⁷-methylguanosine at position 46 (m⁷G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m⁷G46) methyltransferase activity.

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