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Journal of Bacteriology, May 2003, p. 3244-3248, Vol. 185, No. 10
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.10.3244-3248.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Controlled Initiation of Chromosomal Replication in Escherichia coli Requires Functional Hda Protein

Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20007,¹ Department of Cell Biology, Institute for Cancer Research, Det Norske Radiumhospital, Montebello 0310, Oslo, Norway²

Received 20 November 2002/ Accepted 3 March 2003

Regulatory inactivation of DnaA helps ensure that the Escherichia coli chromosome is replicated only once per cell cycle, through accelerated hydrolysis of active replication initiator ATP-DnaA to inactive ADP-DnaA. Analysis of hda strains revealed that the regulatory inactivation of DnaA component Hda is necessary for maintaining controlled initiation but not for cell growth or viability.

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