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Journal of Bacteriology, June 2003, p. 3469-3472, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3469-3472.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Error-Prone Polymerase, DNA Polymerase IV, Is Responsible for Transient Hypermutation during Adaptive Mutation in Escherichia coli

Joshua D. Tompkins, Jennifer L. Nelson, Jill C. Hazel, Stacy L. Leugers, Jeffrey D. Stumpf, and Patricia L. Foster*

Department of Biology, Indiana University, Bloomington, Indiana 47405

Received 31 December 2002/ Accepted 12 March 2003

The frequencies of nonselected mutations among adaptive Lac+ revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.

* Corresponding author. Mailing address: Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN 47405. Phone: (812) 855-4084. Fax: (812) 855-6705. E-mail: plfoster{at}indiana.edu.

Journal of Bacteriology, June 2003, p. 3469-3472, Vol. 185, No. 11
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.11.3469-3472.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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