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Error-Prone Polymerase, DNA Polymerase IV, Is Responsible for Transient Hypermutation during Adaptive Mutation in Escherichia coli

Department of Biology, Indiana University, Bloomington, Indiana 47405

Received 31 December 2002/ Accepted 12 March 2003

The frequencies of nonselected mutations among adaptive Lac⁺ revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.

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