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Journal of Bacteriology, June 2003, p. 3524-3526, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3524-3526.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Protein Synthesis in Escherichia coli with Mischarged tRNA

Bokkee Min,1 Makoto Kitabatake,1 Carla Polycarpo,1 Joanne Pelaschier,1 Gregory Raczniak,1 Benfang Ruan,1 Hiroyuki Kobayashi,1 Suk Namgoong,1 and Dieter Söll1,2*

Departments of Molecular Biophysics and Biochemistry,1 Chemistry, Yale University, New Haven, Connecticut 06520-81142

Received 17 December 2002/ Accepted 2 April 2003

Two types of aspartyl-tRNA synthetase exist: the discriminating enzyme (D-AspRS) forms only Asp-tRNAAsp, while the nondiscriminating one (ND-AspRS) also synthesizes Asp-tRNAAsn, a required intermediate in protein synthesis in many organisms (but not in Escherichia coli). On the basis of the E. coli trpA34 missense mutant transformed with heterologous ND-aspS genes, we developed a system with which to measure the in vivo formation of Asp-tRNAAsn and its acceptance by elongation factor EF-Tu. While large amounts of Asp-tRNAAsn are detrimental to E. coli, smaller amounts support protein synthesis and allow the formation of up to 38% of the wild-type level of missense-suppressed tryptophan synthetase.

* Corresponding author. Mailing address: Department of Molecular Biophysics and Biochemistry, Yale University, P.O. Box 208114, 266 Whitney Ave., New Haven, CT 06520-8114. Phone: (203) 432-6200. Fax: (203) 432-6202. E-mail: soll{at}trna.chem.yale.edu.

Journal of Bacteriology, June 2003, p. 3524-3526, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3524-3526.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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