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 Previous Article

Journal of Bacteriology, June 2003, p. 3678-3682, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3678-3682.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Xenorhabdus nematophila Requires an Intact iscRSUA-hscBA-fdx Operon To Colonize Steinernema carpocapsae Nematodes

Eric C. Martens, Joseph Gawronski-Salerno, Danielle L. Vokal, Molly C. Pellitteri, Megan L. Menard, and Heidi Goodrich-Blair*

Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706

Received 17 October 2002/ Accepted 1 April 2003

An insertion between iscA and hscB of the Xenorhabdus nematophila iscRSUA-hscBA-fdx locus, predicted to encode Fe-S assembly machinery, prevented colonization of Steinernema carpocapsae nematodes. The insertion disrupted cotranscription of iscA and hscB, but did not reduce hscBA expression, suggesting that X. nematophila requires coordinated expression of the isc-hsc-fdx locus for colonization.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin-Madison, E. B. Fred Hall, 1550 Linden Dr., Madison, WI 53706. Phone: (608) 265-4537. Fax: (608) 262-9865. E-mail: hgblair{at}bact.wisc.edu.

Journal of Bacteriology, June 2003, p. 3678-3682, Vol. 185, No. 12
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.12.3678-3682.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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