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Journal of Bacteriology, July 2003, p. 3690-3695, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3690-3695.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutants Suppressing Novobiocin Hypersensitivity of a mukB Null Mutation

Shun Adachi and Sota Hiraga*

Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Konoe, Yoshida, Sakyo, Kyoto 606-8501, Japan

Received 11 March 2003/ Accepted 9 April 2003

The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or near the gyrB gene, and the four tested clones have an amino acid substitution in the DNA gyrase beta subunit. We found that in the mukB mutant, the process of sister chromosome segregation is strikingly hypersensitive to novobiocin; however, the effect of novobiocin on growth, which was measured by culture turbidity, is the same as that of the wild-type strain.

* Corresponding author. Mailing address: Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Konoe, Yoshida, Sakyo, Kyoto 606-8501, Japan. Phone: 81-75-753-4415. Fax: 81-75-753-4419. E-mail: hiraga{at}rg1.rg.med.kyoto-u.ac.jp.

Journal of Bacteriology, July 2003, p. 3690-3695, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3690-3695.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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