This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Minagawa, S.
Right arrow Articles by Utsumi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minagawa, S.
Right arrow Articles by Utsumi, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2003, p. 3696-3702, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3696-3702.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Molecular Characterization of the Mg2+ Stimulon of Escherichia coli

Shu Minagawa,1 Hiroshi Ogasawara,1 Akinori Kato,1 Kaneyoshi Yamamoto,1 Yoko Eguchi,1 Taku Oshima,2 Hirotada Mori,2 Akira Ishihama,3 and Ryutaro Utsumi1*

Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505,1 Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma 630-0101,2 Division of Molecular Biology, Nippon Institute for Biological Science, Ome, Tokyo 198-0024, Japan3

Received 30 October 2002/ Accepted 14 April 2003

Transcription profile microarray analysis in Escherichia coli was performed to identify the member genes of the Mg2+ stimulon that respond to the availability of external Mg2+ in a PhoP/PhoQ two-component system-dependent manner. The mRNA levels of W3110 in the presence of 30 mM MgCl2, WP3022 (phoP defective), and WQ3007 (phoQ defective) were compared with those of W3110 in the absence of MgCl2. The expression ratios of a total of 232 genes were <0.75 in all three strains (the supplemental data are shown at http://www.nara.kindai.ac.jp/nogei/seiken/array.html), suggesting that the PhoP/PhoQ system is involved directly or indirectly in the transcription of these genes. Of those, 26 contained the PhoP box-like sequences with the direct repeats of (T/G)GTTTA within 500 bp upstream of the initiation codon. Furthermore, S1 nuclease assays of 26 promoters were performed to verify six new Mg2+ stimulon genes, hemL, nagA, rstAB, slyB, vboR, and yrbL, in addition to the phoPQ, mgrB, and mgtA genes reported previously. In gel shift and DNase I footprinting assays, all of these genes were found to be regulated directly by PhoP. Thus, we concluded that the phoPQ, mgrB, mgtA, hemL, nagA, rstAB, slyB, vboR, and yrbL genes make up the Mg2+ stimulon in E. coli.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan. Phone: 81-742-43-7273 (ext. 3309). Fax: 81-742-43-1445. E-mail: utsumi{at}nara.kindai.ac.jp.

Journal of Bacteriology, July 2003, p. 3696-3702, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3696-3702.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Choi, E., Groisman, E. A., Shin, D. (2009). Activated by Different Signals, the PhoP/PhoQ Two-Component System Differentially Regulates Metal Uptake. J. Bacteriol. 191: 7174-7181 [Abstract] [Full Text]  
  • Handford, J. I., Ize, B., Buchanan, G., Butland, G. P., Greenblatt, J., Emili, A., Palmer, T. (2009). Conserved Network of Proteins Essential for Bacterial Viability. J. Bacteriol. 191: 4732-4749 [Abstract] [Full Text]  
  • Msadek, T. (2009). Grasping at Shadows: Revealing the Elusive Nature of Essential Genes. J. Bacteriol. 191: 4701-4704 [Full Text]  
  • Yan, Q., Gao, W., Wu, X.-G., Zhang, L.-Q. (2009). Regulation of the PcoI/PcoR quorum-sensing system in Pseudomonas fluorescens 2P24 by the PhoP/PhoQ two-component system. Microbiology 155: 124-133 [Abstract] [Full Text]  
  • Jeon, J., Kim, H., Yun, J., Ryu, S., Groisman, E. A., Shin, D. (2008). RstA-Promoted Expression of the Ferrous Iron Transporter FeoB under Iron-Replete Conditions Enhances Fur Activity in Salmonella enterica. J. Bacteriol. 190: 7326-7334 [Abstract] [Full Text]  
  • Kong, W., Weatherspoon, N., Shi, Y. (2008). Molecular Mechanism for Establishment of Signal-dependent Regulation in the PhoP/PhoQ System. J. Biol. Chem. 283: 16612-16621 [Abstract] [Full Text]  
  • Goldman, S. R., Tu, Y., Goldberg, M. B. (2008). Differential Regulation by Magnesium of the Two MsbB Paralogs of Shigella flexneri. J. Bacteriol. 190: 3526-3537 [Abstract] [Full Text]  
  • Gunasekera, T. S., Csonka, L. N., Paliy, O. (2008). Genome-Wide Transcriptional Responses of Escherichia coli K-12 to Continuous Osmotic and Heat Stresses. J. Bacteriol. 190: 3712-3720 [Abstract] [Full Text]  
  • Eguchi, Y., Itou, J., Yamane, M., Demizu, R., Yamato, F., Okada, A., Mori, H., Kato, A., Utsumi, R. (2007). B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli. Proc. Natl. Acad. Sci. USA 104: 18712-18717 [Abstract] [Full Text]  
  • Cabeza, M. L., Aguirre, A., Soncini, F. C., Vescovi, E. G. (2007). Induction of RpoS Degradation by the Two-Component System Regulator RstA in Salmonella enterica. J. Bacteriol. 189: 7335-7342 [Abstract] [Full Text]  
  • Miyashiro, T., Goulian, M. (2007). Stimulus-dependent differential regulation in the Escherichia coli PhoQ PhoP system. Proc. Natl. Acad. Sci. USA 104: 16305-16310 [Abstract] [Full Text]  
  • Bachhawat, P., Stock, A. M. (2007). Crystal Structures of the Receiver Domain of the Response Regulator PhoP from Escherichia coli in the Absence and Presence of the Phosphoryl Analog Beryllofluoride. J. Bacteriol. 189: 5987-5995 [Abstract] [Full Text]  
  • Kato, A., Mitrophanov, A. Y., Groisman, E. A. (2007). A connector of two-component regulatory systems promotes signal amplification and persistence of expression. Proc. Natl. Acad. Sci. USA 104: 12063-12068 [Abstract] [Full Text]  
  • Ogasawara, H., Hasegawa, A., Kanda, E., Miki, T., Yamamoto, K., Ishihama, A. (2007). Genomic SELEX Search for Target Promoters under the Control of the PhoQP-RstBA Signal Relay Cascade. J. Bacteriol. 189: 4791-4799 [Abstract] [Full Text]  
  • Campbell, T. L., Ederer, C. S., Allali-Hassani, A., Brown, E. D. (2007). Isolation of the rstA Gene as a Multicopy Suppressor of YjeE, an Essential ATPase of Unknown Function in Escherichia coli. J. Bacteriol. 189: 3318-3321 [Abstract] [Full Text]  
  • Wu, H., Mao, F., Olman, V., Xu, Y. (2007). Hierarchical classification of functionally equivalent genes in prokaryotes. Nucleic Acids Res 35: 2125-2140 [Abstract] [Full Text]  
  • Golubeva, Y. A., Slauch, J. M. (2006). Salmonella enterica Serovar Typhimurium Periplasmic Superoxide Dismutase SodCI Is a Member of the PhoPQ Regulon and Is Induced in Macrophages. J. Bacteriol. 188: 7853-7861 [Abstract] [Full Text]  
  • Tu, X., Latifi, T., Bougdour, A., Gottesman, S., Groisman, E. A. (2006). The PhoP/PhoQ two-component system stabilizes the alternative sigma factor RpoS in Salmonella enterica. Proc. Natl. Acad. Sci. USA 103: 13503-13508 [Abstract] [Full Text]  
  • Venkatesh, B., Babujee, L., Liu, H., Hedley, P., Fujikawa, T., Birch, P., Toth, I., Tsuyumu, S. (2006). The Erwinia chrysanthemi 3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants.. J. Bacteriol. 188: 3088-3098 [Abstract] [Full Text]  
  • GROISMAN, E.A., CROMIE, M.J., SHI, Y., LATIFI, T. (2006). A Mg2+-responding RNA That Controls the Expression of a Mg2+ Transporter. Cold Spring Harb Symp Quant Biol 71: 251-258 [Abstract]  
  • Perron-Savard, P., De Crescenzo, G., Moual, H. L. (2005). Dimerization and DNA binding of the Salmonella enterica PhoP response regulator are phosphorylation independent. Microbiology 151: 3979-3987 [Abstract] [Full Text]  
  • Zwir, I., Huang, H., Groisman, E. A. (2005). Analysis of differentially-regulated genes within a regulatory network by GPS genome navigation. Bioinformatics 21: 4073-4083 [Abstract] [Full Text]  
  • Sermon, J., Wevers, E. M.-R. P., Jansen, L., De Spiegeleer, P., Vanoirbeek, K., Aertsen, A., Michiels, C. W. (2005). CorA Affects Tolerance of Escherichia coli and Salmonella enterica Serovar Typhimurium to the Lactoperoxidase Enzyme System but Not to Other Forms of Oxidative Stress. Appl. Environ. Microbiol. 71: 6515-6523 [Abstract] [Full Text]  
  • Yamamoto, K., Ishihama, A. (2005). Transcriptional Response of Escherichia coli to External Zinc. J. Bacteriol. 187: 6333-6340 [Abstract] [Full Text]  
  • Batchelor, E., Walthers, D., Kenney, L. J., Goulian, M. (2005). The Escherichia coli CpxA-CpxR Envelope Stress Response System Regulates Expression of the Porins OmpF and OmpC. J. Bacteriol. 187: 5723-5731 [Abstract] [Full Text]  
  • Llama-Palacios, A., Lopez-Solanilla, E., Rodriguez-Palenzuela, P. (2005). Role of the PhoP-PhoQ System in the Virulence of Erwinia chrysanthemi Strain 3937: Involvement in Sensitivity to Plant Antimicrobial Peptides, Survival at Acid pH, and Regulation of Pectolytic Enzymes. J. Bacteriol. 187: 2157-2162 [Abstract] [Full Text]  
  • Zwir, I., Shin, D., Kato, A., Nishino, K., Latifi, T., Solomon, F., Hare, J. M., Huang, H., Groisman, E. A. (2005). Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proc. Natl. Acad. Sci. USA 102: 2862-2867 [Abstract] [Full Text]  
  • Shin, D., Groisman, E. A. (2005). Signal-dependent Binding of the Response Regulators PhoP and PmrA to Their Target Promoters in Vivo. J. Biol. Chem. 280: 4089-4094 [Abstract] [Full Text]  
  • Bringer, M.-A., Barnich, N., Glasser, A.-L., Bardot, O., Darfeuille-Michaud, A. (2005). HtrA Stress Protein Is Involved in Intramacrophagic Replication of Adherent and Invasive Escherichia coli Strain LF82 Isolated from a Patient with Crohn's Disease. Infect. Immun. 73: 712-721 [Abstract] [Full Text]  
  • Kitagawa, M., Ara, T., Arifuzzaman, M., Ioka-Nakamichi, T., Inamoto, E., Toyonaga, H., Mori, H. (2005). Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-299 [Abstract] [Full Text]  
  • Ogasawara, H., Teramoto, J., Hirao, K., Yamamoto, K., Ishihama, A., Utsumi, R. (2004). Negative Regulation of DNA Repair Gene (ung) Expression by the CpxR/CpxA Two-Component System in Escherichia coli K-12 and Induction of Mutations by Increased Expression of CpxR. J. Bacteriol. 186: 8317-8325 [Abstract] [Full Text]  
  • Shi, Y., Latifi, T., Cromie, M. J., Groisman, E. A. (2004). Transcriptional Control of the Antimicrobial Peptide Resistance ugtL Gene by the Salmonella PhoP and SlyA Regulatory Proteins. J. Biol. Chem. 279: 38618-38625 [Abstract] [Full Text]  
  • Bordi, C., Ansaldi, M., Gon, S., Jourlin-Castelli, C., Iobbi-Nivol, C., Mejean, V. (2004). Genes Regulated by TorR, the Trimethylamine Oxide Response Regulator of Shewanella oneidensis. J. Bacteriol. 186: 4502-4509 [Abstract] [Full Text]  
  • Eguchi, Y., Okada, T., Minagawa, S., Oshima, T., Mori, H., Yamamoto, K., Ishihama, A., Utsumi, R. (2004). Signal Transduction Cascade between EvgA/EvgS and PhoP/PhoQ Two-Component Systems of Escherichia coli. J. Bacteriol. 186: 3006-3014 [Abstract] [Full Text]  
  • Lejona, S., Castelli, M. E., Cabeza, M. L., Kenney, L. J., Garcia Vescovi, E., Soncini, F. C. (2004). PhoP Can Activate Its Target Genes in a PhoQ-Independent Manner. J. Bacteriol. 186: 2476-2480 [Abstract] [Full Text]  
  • Mallik, P., Pratt, T. S., Beach, M. B., Bradley, M. D., Undamatla, J., Osuna, R. (2004). Growth Phase-Dependent Regulation and Stringent Control of fis Are Conserved Processes in Enteric Bacteria and Involve a Single Promoter (fis P) in Escherichia coli. J. Bacteriol. 186: 122-135 [Abstract] [Full Text]  
  • Mouslim, C., Latifi, T., Groisman, E. A. (2003). Signal-dependent Requirement for the Co-activator Protein RcsA in Transcription of the RcsB-regulated ugd Gene. J. Biol. Chem. 278: 50588-50595 [Abstract] [Full Text]  
  • Lejona, S., Aguirre, A., Cabeza, M. L., Vescovi, E. G., Soncini, F. C. (2003). Molecular Characterization of the Mg2+-Responsive PhoP-PhoQ Regulon in Salmonella enterica. J. Bacteriol. 185: 6287-6294 [Abstract] [Full Text]  
  • Eguchi, Y., Oshima, T., Mori, H., Aono, R., Yamamoto, K., Ishihama, A., Utsumi, R. (2003). Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli. Microbiology 149: 2819-2828 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»