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Journal of Bacteriology, July 2003, p. 3726-3734, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3726-3734.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Probing the Catalytic Activity of a Cell Division-Specific Transpeptidase In Vivo with ß-Lactams

Christian Eberhardt,{dagger} Lars Kuerschner,{ddagger} and David S. Weiss*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 24 February 2003/ Accepted 21 April 2003

Penicillin-binding protein 3 (PBP3; also called FtsI) is a transpeptidase that catalyzes cross-linking of the peptidoglycan cell wall in the division septum of Escherichia coli. To determine whether the catalytic activity of PBP3 is activated during division, we assayed acylation of PBP3 with three ß-lactams (cephalexin, aztreonam, and piperacillin) in growing cells. Acylation of PBP3 with cephalexin, but not aztreonam or piperacillin, appeared to be stimulated by cell division. Specifically, cephalexin acylated PBP3 about 50% faster in a population of dividing cells than in a population of filamentous cells in which division was inhibited by inactivation or depletion of FtsZ, FtsA, FtsQ, FtsW, or FtsN. However, in a simpler in vitro system using isolated membranes, acylation with cephalexin was not impaired by depletion of FtsW or FtsN. A conflicting previous report that the ftsA3(Ts) allele interferes with acylation of PBP3 was found to be due to the presence of a thermolabile PBP3 in the strain used in that study. The new findings presented here are discussed in light of the hypothesis that the catalytic activity of PBP3 is stimulated by interaction(s) with other division proteins. We suggest that there might be allosteric activation of substrate binding.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7785. Fax: (319) 335-9006. E-mail: david-weiss{at}uiowa.edu.

{dagger} Present address: Institut für Medizinische Mikrobiologie, Universitätsklinikum Tübingen, 72076 Tübingen, Germany.

{ddagger} Present address: Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.

Journal of Bacteriology, July 2003, p. 3726-3734, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3726-3734.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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