This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kersulyte, D.
Right arrow Articles by Berg, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kersulyte, D.
Right arrow Articles by Berg, D. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2003, p. 3764-3772, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3764-3772.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cluster of Type IV Secretion Genes in Helicobacter pylori's Plasticity Zone

Dangeruta Kersulyte,1 Billie Velapatiño,1,2 Asish K. Mukhopadhyay,1,{dagger} Lizbeth Cahuayme,2 Alejandro Bussalleu,3 Juan Combe,3 Robert H. Gilman,2 and Douglas E. Berg1*

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110,1 Department of Microbiology,2 Department of Gastroenterology, Facultad de Medecina, Universidad Peruana Cayetano Heredia, Lima 31, Peru3

Received 21 February 2003/ Accepted 21 April 2003

Some genes present in only certain strains of the genetically diverse gastric pathogen Helicobacter pylori may affect its phenotype and/or evolutionary potential. Here we describe a new 16.3-kb segment, 7 of whose 16 open reading frames are homologs of type IV secretion genes (virB4, virB7 to virB11, and virD4), the third such putative secretion gene cluster found in H. pylori. This segment, to be called tfs3, was discovered by subtractive hybridization and chromosome walking. Full-length and truncated tfs3 elements were found in 20 and 19%, respectively, of 94 strains tested, which were from Spain, Peru, India, and Japan. A tfs3 remnant (6 kb) was found in an archived stock of reference strain J99, although it was not included in this strain's published genome sequence. PCR and DNA sequence analyses indicated the following. (i) tfs3's ends are conserved. (ii) Right-end insertion occurred at one specific site in a chromosomal region that is varied in gene content and arrangement, the "plasticity zone." (iii) Left-end insertion occurred at different sites in each of nine strains studied. (iv) Sequences next to the right-end target in tfs3-free strains were absent from most strains carrying full-length tfs3 elements. These patterns suggested insertion by a transposition-like event, but one in which targets are chosen with little or no specificity at the left end and high specificity at the right end, thereby deleting the intervening DNA.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Campus Box 8230, Washington University School of Medicine, St. Louis, MO 63110. Phone: (314) 362-2772. Fax: (314) 362-1232. E-mail: berg{at}borcim.wustl.edu.

{dagger} Present address: National Institute of Cholera and Enteric Diseases, Scheme-XM, Beliaghata, Calcutta-700010, India.

Journal of Bacteriology, July 2003, p. 3764-3772, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3764-3772.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Romo-Gonzalez, C., Salama, N. R., Burgeno-Ferreira, J., Ponce-Castaneda, V., Lazcano-Ponce, E., Camorlinga-Ponce, M., Torres, J. (2009). Differences in Genome Content among Helicobacter pylori Isolates from Patients with Gastritis, Duodenal Ulcer, or Gastric Cancer Reveal Novel Disease-Associated Genes. Infect. Immun. 77: 2201-2211 [Abstract] [Full Text]  
  • Castillo, A. R., Woodruff, A. J., Connolly, L. E., Sause, W. E., Ottemann, K. M. (2008). Recombination-Based In Vivo Expression Technology Identifies Helicobacter pylori Genes Important for Host Colonization. Infect. Immun. 76: 5632-5644 [Abstract] [Full Text]  
  • Yamaoka, Y. (2008). Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis. J Med Microbiol 57: 545-553 [Abstract] [Full Text]  
  • Kutter, S., Buhrdorf, R., Haas, J., Schneider-Brachert, W., Haas, R., Fischer, W. (2008). Protein Subassemblies of the Helicobacter pylori Cag Type IV Secretion System Revealed by Localization and Interaction Studies. J. Bacteriol. 190: 2161-2171 [Abstract] [Full Text]  
  • Alvi, A., Devi, S. M., Ahmed, I., Hussain, M. A., Rizwan, M., Lamouliatte, H., Megraud, F., Ahmed, N. (2007). Microevolution of Helicobacter pylori Type IV Secretion Systems in an Ulcer Disease Patient over a Ten-Year Period. J. Clin. Microbiol. 45: 4039-4043 [Abstract] [Full Text]  
  • Oyarzabal, O. A., Rad, R., Backert, S. (2007). Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni. J. Clin. Microbiol. 45: 402-408 [Abstract] [Full Text]  
  • Oleastro, M., Monteiro, L., Lehours, P., Megraud, F., Menard, A. (2006). Identification of Markers for Helicobacter pylori Strains Isolated from Children with Peptic Ulcer Disease by Suppressive Subtractive Hybridization. Infect. Immun. 74: 4064-4074 [Abstract] [Full Text]  
  • Karnholz, A., Hoefler, C., Odenbreit, S., Fischer, W., Hofreuter, D., Haas, R. (2006). Functional and Topological Characterization of Novel Components of the comB DNA Transformation Competence System in Helicobacter pylori. J. Bacteriol. 188: 882-893 [Abstract] [Full Text]  
  • Backert, S., Kwok, T., Konig, W. (2005). Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins. Microbiology 151: 3493-3503 [Abstract] [Full Text]  
  • Larsen, J. C., Szymanski, C., Guerry, P. (2004). N-Linked Protein Glycosylation Is Required for Full Competence in Campylobacter jejuni 81-176. J. Bacteriol. 186: 6508-6514 [Abstract] [Full Text]  
  • Batchelor, R. A., Pearson, B. M., Friis, L. M., Guerry, P., Wells, J. M. (2004). Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species. Microbiology 150: 3507-3517 [Abstract] [Full Text]  
  • Engledow, A. S., Medrano, E. G., Mahenthiralingam, E., LiPuma, J. J., Gonzalez, C. F. (2004). Involvement of a Plasmid-Encoded Type IV Secretion System in the Plant Tissue Watersoaking Phenotype of Burkholderia cenocepacia. J. Bacteriol. 186: 6015-6024 [Abstract] [Full Text]  
  • Terradot, L., Durnell, N., Li, M., Li, M., Ory, J., Labigne, A., Legrain, P., Colland, F., Waksman, G. (2004). Biochemical Characterization of Protein Complexes from the Helicobacter pylori Protein Interaction Map: Strategies for Complex Formation and Evidence for Novel Interactions Within Type IV Secretion Systems. Mol. Cell. Proteomics 3: 809-819 [Abstract] [Full Text]  
  • Lehours, P, Dupouy, S, Bergey, B, Ruskone-Foumestraux, A, Delchier, J C, Rad, R, Richy, F, Tankovic, J, Zerbib, F, Megraud, F, Menard, A (2004). Identification of a genetic marker of Helicobacter pylori strains involved in gastric extranodal marginal zone B cell lymphoma of the MALT-type. Gut 53: 931-937 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»