This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fiorentino, G.
Right arrow Articles by Bartolucci, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fiorentino, G.
Right arrow Articles by Bartolucci, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2003, p. 3926-3934, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3926-3934.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcriptional Regulation of the Gene Encoding an Alcohol Dehydrogenase in the Archaeon Sulfolobus solfataricus Involves Multiple Factors and Control Elements

Gabriella Fiorentino,1 Raffaele Cannio,2 Mosè Rossi,1,2 and Simonetta Bartolucci1*

Dipartimento di Chimica Biologica, Università degli Studi di Napoli Federico II,1 Istituto di Biochimica delle Proteine, CNR, Naples, Italy2

Received 4 November 2002/ Accepted 7 April 2003

A transcriptionally active region has been identified in the 5' flanking region of the alcohol dehydrogenase gene of the crenarchaeon Sulfolobus solfataricus through the evaluation of the activity of putative transcriptional regulators and the role of the region upstream of the gene under specific metabolic circumstances. Electrophoretic mobility shift assays with crude extracts revealed protein complexes that most likely contain TATA box-associated factors. When the TATA element was deleted from the region, binding sites for both DNA binding proteins, such as the small chromatin structure-modeling Sso7d and Sso10b (Alba), and transcription factors, such as the repressor Lrs14, were revealed. To understand the molecular mechanisms underlying the substrate-induced expression of the adh gene, the promoter was analyzed for the presence of cis-acting elements recognized by specific transcription factors upon exposure of the cell to benzaldehyde. Progressive dissection of the identified promoter region restricted the analysis to a minimal responsive element (PAL) located immediately upstream of the transcription factor B-responsive element-TATA element, resembling typical bacterial regulatory sequences. A benzaldehyde-activated transcription factor (Bald) that specifically binds to the PAL cis-acting element was also identified. This protein was purified from heparin-fractionated extracts of benzaldehyde-induced cells and was shown to have a molecular mass of ~16 kDa. The correlation between S. solfataricus adh gene activation and benzaldehyde-inducible occupation of a specific DNA sequence in its promoter suggests that a molecular signaling mechanism is responsible for the switch of the aromatic aldehyde metabolism as a response to environmental changes.

* Corresponding author. Mailing address: Dipartimento di Chimica Biologica, Università degli Studi di Napoli Federico II, Via Mezzocannone 16-80134, Naples, Italy. Phone: 39-0812534732. Fax: 39-0812534614. E-mail: bartoluc{at}unina.it.

Journal of Bacteriology, July 2003, p. 3926-3934, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3926-3934.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kumarevel, T., Nakano, N., Ponnuraj, K., Gopinath, S. C. B., Sakamoto, K., Shinkai, A., Kumar, P. K. R., Yokoyama, S. (2008). Crystal structure of glutamine receptor protein from Sulfolobus tokodaii strain 7 in complex with its effector L-glutamine: implications of effector binding in molecular association and DNA binding. Nucleic Acids Res 36: 4808-4820 [Abstract] [Full Text]  
  • Lu, Q., Han, J., Zhou, L., Coker, J. A., DasSarma, P., DasSarma, S., Xiang, H. (2008). Dissection of the regulatory mechanism of a heat-shock responsive promoter in Haloarchaea: a new paradigm for general transcription factor directed archaeal gene regulation. Nucleic Acids Res 36: 3031-3042 [Abstract] [Full Text]  
  • Fiorentino, G., Ronca, R., Cannio, R., Rossi, M., Bartolucci, S. (2007). MarR-Like Transcriptional Regulator Involved in Detoxification of Aromatic Compounds in Sulfolobus solfataricus. J. Bacteriol. 189: 7351-7360 [Abstract] [Full Text]  
  • Marsh, V. L., Peak-Chew, S. Y., Bell, S. D. (2005). Sir2 and the Acetyltransferase, Pat, Regulate the Archaeal Chromatin Protein, Alba. J. Biol. Chem. 280: 21122-21128 [Abstract] [Full Text]  
  • Xie, Y., Reeve, J. N. (2004). Transcription by Methanothermobacter thermautotrophicus RNA Polymerase In Vitro Releases Archaeal Transcription Factor B but Not TATA-Box Binding Protein from the Template DNA. J. Bacteriol. 186: 6306-6310 [Abstract] [Full Text]  
  • Lou, H., Duan, Z., Huo, X., Huang, L. (2004). Modulation of Hyperthermophilic DNA Polymerase Activity by Archaeal Chromatin Proteins. J. Biol. Chem. 279: 127-132 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»