This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jolivet, E.
Right arrow Articles by Myllykallio, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jolivet, E.
Right arrow Articles by Myllykallio, H.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2003, p. 3958-3961, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3958-3961.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Physiological Responses of the Hyperthermophilic Archaeon "Pyrococcus abyssi" to DNA Damage Caused by Ionizing Radiation

Edmond Jolivet,1* Fujihiko Matsunaga,2 Yoshizumi Ishino,3 Patrick Forterre,2 Daniel Prieur,1 and Hannu Myllykallio2

Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne occidentale (UBO), CNRS UMR 6539, Technopôle Brest-Iroise, 29 280 Plouzané,1 Institut de Génétique et de Microbiologie (IGM), Université Paris-Sud (UPS), CNRS UMR 8621, 91 405 Orsay Cedex, France,2 Laboratory of Protein Chemistry & Engineering Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka-shi, Fukuoka 812-8581, Japan3

Received 10 December 2002/ Accepted 18 March 2003

The mechanisms by which hyperthermophilic Archaea, such as "Pyrococcus abyssi" and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of "P. abyssi" chromosomes took place within chromosome fragmentation. DNA synthesis in irradiated "P. abyssi" cells during the DNA repair phase was inhibited in comparison to nonirradiated control cultures, suggesting that DNA damage causes a replication block in this organism. We also found evidence for transient export of damaged DNA out of irradiated "P. abyssi" cells prior to a restart of chromosomal DNA synthesis. Our cell fractionation assays further suggest that "P. abyssi" contains a highly efficient DNA repair system which is continuously ready to repair the DNA damage caused by high temperature and/or ionizing radiation.

* Corresponding author. Mailing address: Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne occidentale (UBO), CNRS UMR 6539, Technopôle Brest-Iroise, Place Nicolas Copernic, 29 280 Plouzané, France. Phone: (33) 2 98 49 87 79. Fax: (33) 2 98 49 87 05. E-mail: jolivet{at}univ-brest.fr.

Journal of Bacteriology, July 2003, p. 3958-3961, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3958-3961.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»