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Journal of Bacteriology, July 2003, p. 3972-3977, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3972-3977.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The dinB Operon and Spontaneous Mutation in Escherichia coli

Gregory J. McKenzie,1,{dagger} Daniel B. Magner,2 Peter L. Lee,1,{ddagger} and Susan M. Rosenberg1,2,3,4*

Department of Molecular and Human Genetics,1 Interdepartmental Graduate Program in Cell and Molecular Biology,2 Departments of Biochemistry and Molecular Biology,3 Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030-34114

Received 30 September 2002/ Accepted 9 April 2003

Apparently conflicting data regarding the role of SOS-inducible, error-prone DNA polymerase IV (DinB) in spontaneous mutation are resolved by the finding that mutation is reduced by a polar allele with which dinB and neighboring yafN are deleted but not by two nonpolar dinB alleles. We demonstrate the existence of a dinB operon that contains four genes, dinB-yafN-yafO-yafP. The results imply a role for yafN, yafO, and/or yafP in spontaneous mutation.

* Corresponding author. Mailing address: Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Rm. S809A, Mail Stop BCM225, Houston, TX 77030-3411. Phone: (713) 798-6924. Fax: (713) 798-8967. E-mail: smr{at}bcm.tmc.edu.

{dagger} Present address: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

{ddagger} Present address: Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005.

Journal of Bacteriology, July 2003, p. 3972-3977, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3972-3977.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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