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The dinB Operon and Spontaneous Mutation in Escherichia coli

Gregory J. McKenzie,^1, Daniel B. Magner,² Peter L. Lee,^1, and Susan M. Rosenberg^1,2,3,4*

Department of Molecular and Human Genetics,¹ Interdepartmental Graduate Program in Cell and Molecular Biology,² Departments of Biochemistry and Molecular Biology,³ Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030-3411⁴

Received 30 September 2002/ Accepted 9 April 2003

Apparently conflicting data regarding the role of SOS-inducible, error-prone DNA polymerase IV (DinB) in spontaneous mutation are resolved by the finding that mutation is reduced by a polar allele with which dinB and neighboring yafN are deleted but not by two nonpolar dinB alleles. We demonstrate the existence of a dinB operon that contains four genes, dinB-yafN-yafO-yafP. The results imply a role for yafN, yafO, and/or yafP in spontaneous mutation.

Present address: Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Present address: Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005.

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