Journal of Bacteriology, July 2003, p. 3978-3982, Vol. 185, No. 13
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.13.3978-3982.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Bradyrhizobium japonicum NnrR, a Denitrification Regulator, Expands the FixLJ-FixK2 Regulatory Cascade
Socorro Mesa,1* Eulogio J. Bedmar,2 Astrid Chanfon,1 Hauke Hennecke,1 and Hans-Martin Fischer1
Institute of Microbiology, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland,1
Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080 Granada, Spain2
Received 21 January 2003/
Accepted 16 April 2003
In Bradyrhizobium japonicum, a gene named nnrR was identified which encodes a protein with high similarity to FNR/CRP-type transcriptional regulators. Mutant strains carrying an nnrR null mutation were unable to grow anaerobically in the presence of nitrate or nitrite, and they lacked both nitrate and nitrite reductase activities. Anaerobic activation of an nnrR'-'lacZ fusion required FixLJ and FixK2. In turn, N oxide-mediated induction of nir and nor genes encoding nitrite and nitric oxide reductase, respectively, depended on NnrR. Thus, NnrR expands the FixLJ-FixK2 regulatory cascade by an additional control level which integrates the N oxide signal required for maximal induction of the denitrification genes.
* Corresponding author. Mailing address: Institute of Microbiology, Eidgenössische Technische Hochschule, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland. Phone: 41-1-632-32-83. Fax: 41-1-632-11-48. E-mail: mesam{at}micro.biol.ethz.ch.
Journal of Bacteriology, July 2003, p. 3978-3982, Vol. 185, No. 13
0021-9193/03/$08.00+0 DOI: 10.1128/JB.185.13.3978-3982.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Baek, S.-H., Hartsock, A., Shapleigh, J. P.
(2008). Agrobacterium tumefaciens C58 Uses ActR and FnrN To Control nirK and nor Expression. J. Bacteriol.
190: 78-86
[Abstract]
[Full Text]
-
Lindemann, A., Moser, A., Pessi, G., Hauser, F., Friberg, M., Hennecke, H., Fischer, H.-M.
(2007). New Target Genes Controlled by the Bradyrhizobium japonicum Two-Component Regulatory System RegSR. J. Bacteriol.
189: 8928-8943
[Abstract]
[Full Text]
-
Meakin, G. E., Bueno, E., Jepson, B., Bedmar, E. J., Richardson, D. J., Delgado, M. J.
(2007). The contribution of bacteroidal nitrate and nitrite reduction to the formation of nitrosylleghaemoglobin complexes in soybean root nodules. Microbiology
153: 411-419
[Abstract]
[Full Text]
-
Bobik, C., Meilhoc, E., Batut, J.
(2006). FixJ: a Major Regulator of the Oxygen Limitation Response and Late Symbiotic Functions of Sinorhizobium meliloti. J. Bacteriol.
188: 4890-4902
[Abstract]
[Full Text]
-
Lee, Y.-Y., Shearer, N., Spiro, S.
(2006). Transcription factor NNR from Paracoccus denitrificans is a sensor of both nitric oxide and oxygen: isolation of nnr* alleles encoding effector-independent proteins and evidence for a haem-based sensing mechanism.. Microbiology
152: 1461-1470
[Abstract]
[Full Text]
-
Pauly, N., Pucciariello, C., Mandon, K., Innocenti, G., Jamet, A., Baudouin, E., Herouart, D., Frendo, P., Puppo, A.
(2006). Reactive oxygen and nitrogen species and glutathione: key players in the legume-Rhizobium symbiosis. J Exp Bot
57: 1769-1776
[Abstract]
[Full Text]
-
Sameshima-Saito, R., Chiba, K., Hirayama, J., Itakura, M., Mitsui, H., Eda, S., Minamisawa, K.
(2006). Symbiotic Bradyrhizobium japonicum Reduces N2O Surrounding the Soybean Root System via Nitrous Oxide Reductase. Appl. Environ. Microbiol.
72: 2526-2532
[Abstract]
[Full Text]
-
Conlan, S., Lawrence, C., McCue, L. A.
(2005). Rhodopseudomonas palustris Regulons Detected by Cross-Species Analysis of Alphaproteobacterial Genomes. Appl. Environ. Microbiol.
71: 7442-7452
[Abstract]
[Full Text]
-
Crosson, S., McGrath, P. T., Stephens, C., McAdams, H. H., Shapiro, L.
(2005). Conserved modular design of an oxygen sensory/signaling network with species-specific output. Proc. Natl. Acad. Sci. USA
102: 8018-8023
[Abstract]
[Full Text]
-
Mesa, S., Ucurum, Z., Hennecke, H., Fischer, H.-M.
(2005). Transcription Activation In Vitro by the Bradyrhizobium japonicum Regulatory Protein FixK2. J. Bacteriol.
187: 3329-3338
[Abstract]
[Full Text]
-
Rajashekara, G., Glasner, J. D., Glover, D. A., Splitter, G. A.
(2004). Comparative Whole-Genome Hybridization Reveals Genomic Islands in Brucella Species. J. Bacteriol.
186: 5040-5051
[Abstract]
[Full Text]
-
Delgado, M. J., Bonnard, N., Tresierra-Ayala, A., Bedmar, E. J., Muller, P.
(2003). The Bradyrhizobium japonicum napEDABC genes encoding the periplasmic nitrate reductase are essential for nitrate respiration. Microbiology
149: 3395-3403
[Abstract]
[Full Text]
-
Sciotti, M.-A., Chanfon, A., Hennecke, H., Fischer, H.-M.
(2003). Disparate Oxygen Responsiveness of Two Regulatory Cascades That Control Expression of Symbiotic Genes in Bradyrhizobium japonicum. J. Bacteriol.
185: 5639-5642
[Abstract]
[Full Text]
Copyright © 2003 by the American Society for Microbiology. All rights reserved.