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Bradyrhizobium japonicum NnrR, a Denitrification Regulator, Expands the FixLJ-FixK₂ Regulatory Cascade

Institute of Microbiology, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland,¹ Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080 Granada, Spain²

Received 21 January 2003/ Accepted 16 April 2003

In Bradyrhizobium japonicum, a gene named nnrR was identified which encodes a protein with high similarity to FNR/CRP-type transcriptional regulators. Mutant strains carrying an nnrR null mutation were unable to grow anaerobically in the presence of nitrate or nitrite, and they lacked both nitrate and nitrite reductase activities. Anaerobic activation of an nnrR'-'lacZ fusion required FixLJ and FixK₂. In turn, N oxide-mediated induction of nir and nor genes encoding nitrite and nitric oxide reductase, respectively, depended on NnrR. Thus, NnrR expands the FixLJ-FixK₂ regulatory cascade by an additional control level which integrates the N oxide signal required for maximal induction of the denitrification genes.

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