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Journal of Bacteriology, July 2003, p. 3978-3982, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3978-3982.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Bradyrhizobium japonicum NnrR, a Denitrification Regulator, Expands the FixLJ-FixK2 Regulatory Cascade

Socorro Mesa,1* Eulogio J. Bedmar,2 Astrid Chanfon,1 Hauke Hennecke,1 and Hans-Martin Fischer1

Institute of Microbiology, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland,1 Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080 Granada, Spain2

Received 21 January 2003/ Accepted 16 April 2003

In Bradyrhizobium japonicum, a gene named nnrR was identified which encodes a protein with high similarity to FNR/CRP-type transcriptional regulators. Mutant strains carrying an nnrR null mutation were unable to grow anaerobically in the presence of nitrate or nitrite, and they lacked both nitrate and nitrite reductase activities. Anaerobic activation of an nnrR'-'lacZ fusion required FixLJ and FixK2. In turn, N oxide-mediated induction of nir and nor genes encoding nitrite and nitric oxide reductase, respectively, depended on NnrR. Thus, NnrR expands the FixLJ-FixK2 regulatory cascade by an additional control level which integrates the N oxide signal required for maximal induction of the denitrification genes.

* Corresponding author. Mailing address: Institute of Microbiology, Eidgenössische Technische Hochschule, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland. Phone: 41-1-632-32-83. Fax: 41-1-632-11-48. E-mail: mesam{at}micro.biol.ethz.ch.

Journal of Bacteriology, July 2003, p. 3978-3982, Vol. 185, No. 13
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.13.3978-3982.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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