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Journal of Bacteriology, July 2003, p. 4285-4289, Vol. 185, No. 14
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.14.4285-4289.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

An Integrated Structural and Computational Study of the Thermostability of Two Thioredoxin Mutants from Alicyclobacillus acidocaldarius

Simonetta Bartolucci,1 Giuseppina De Simone,2 Stefania Galdiero,1 Roberto Improta,2 Valeria Menchise,2 Carlo Pedone,2 Emilia Pedone,2 and Michele Saviano2*

Dipartimento di Chimica Biologica, University of Naples "Federico II,",1 Istituto di Biostrutture e Bioimmagini-CNR, 80134 Naples, Italy2

Received 21 February 2003/ Accepted 3 April 2003

We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.

* Corresponding author. Mailing address: Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone 6, 80134 Naples, Italy. Phone: 39-081-2536651. Fax: 39-081-5514305. E-mail: saviano{at}chemistry.unina.it.

Journal of Bacteriology, July 2003, p. 4285-4289, Vol. 185, No. 14
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.14.4285-4289.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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