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Journal of Bacteriology, August 2003, p. 4638-4643, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4638-4643.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Microarray Transcription Analysis of Clinical Staphylococcus aureus Isolates Resistant to Vancomycin

Emmanuel Mongodin,1,{dagger} Jon Finan,1,2 Michael W. Climo,1,3 Adriana Rosato,1 Steven Gill,4 and Gordon L. Archer1,2*

Departments of Medicine,1 Microbiology/Immunology, Medical College of Virginia at Virginia Commonwealth University,2 the Hunter Holmes McGuire Veterans Affairs Medical Center, Richmond, Virginia,3 The Institute for Genomic Research, Rockville, Maryland4

Received 7 February 2003/ Accepted 5 April 2003

The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 µg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 µg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.

* Corresponding author. Mailing address: Division of Infectious Diseases, Virginia Commonwealth University Health System, 1101 East Marshall St., Box 980049, Richmond, VA 23298. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail: garcher{at}hsc.vcu.edu.

{dagger} Present address: The Institute for Genomic Research, Rockville, MD 20850.

Journal of Bacteriology, August 2003, p. 4638-4643, Vol. 185, No. 15
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.15.4638-4643.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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