This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rojas, A. Articles by Ramos, J. L. Search for Related Content PubMed PubMed Citation Articles by Rojas, A. Articles by Ramos, J. L.

In Vivo and In Vitro Evidence that TtgV Is the Specific Regulator of the TtgGHI Multidrug and Solvent Efflux Pump of Pseudomonas putida

Department of Plant Biochemistry and Molecular and Cellular Biology, Consejo Superior de Investigaciones Cientificas, Estación Experimental del Zaidín, E-18008 Granada, Spain

Received 28 February 2003/ Accepted 16 May 2003

The TtgGHI efflux pump of Pseudomonas putida DOT-T1E plays a key role in the innate and induced tolerance of this strain to aromatic hydrocarbons and antibiotics. The ttgGHI operon is expressed constitutively from two overlapping promoters in the absence of solvents and at a higher level in their presence, but not in response to antibiotics. Adjacent to the ttgGHI operon is the divergently transcribed ttgVW operon. In TtgV-deficient backgrounds, although not in a TtgW-deficient background, expression of the ttgGHI and ttgVW operons increased fourfold. This suggests that TtgV represses expression from the ttgG promoters and controls its own. TtgW plays no major role in the regulation of expression of these promoters. Primer extension revealed that the divergent ttgG and ttgV promoters overlap, and mobility shift assays indicated that TtgV binds to this region with high affinity. DNaseI footprint assays revealed that TtgV protected four DNA helical turns that include the -10 and -35 boxes of the ttgV and ttgG promoters.

This article has been cited by other articles:

• Busch, A., Lacal, J., Martos, A., Ramos, J. L., Krell, T. (2007). Bacterial sensor kinase TodS interacts with agonistic and antagonistic signals. Proc. Natl. Acad. Sci. USA 104: 13774-13779 [Abstract] [Full Text]  
• Guazzaroni, M.-E., Gallegos, M.-T., Ramos, J. L., Krell, T. (2007). Different Modes of Binding of Mono- and Biaromatic Effectors to the Transcriptional Regulator TTGV: ROLE IN DIFFERENTIAL DEREPRESSION FROM ITS COGNATE OPERATOR. J. Biol. Chem. 282: 16308-16316 [Abstract] [Full Text]  
• Duque, E., Rodriguez-Herva, J.-J., de la Torre, J., Dominguez-Cuevas, P., Munoz-Rojas, J., Ramos, J.-L. (2007). The RpoT Regulon of Pseudomonas putida DOT-T1E and Its Role in Stress Endurance against Solvents. J. Bacteriol. 189: 207-219 [Abstract] [Full Text]  
• Wang, N., Lu, S.-E., Records, A. R., Gross, D. C. (2006). Characterization of the Transcriptional Activators SalA and SyrF, Which Are Required for Syringomycin and Syringopeptin Production by Pseudomonas syringae pv. syringae. J. Bacteriol. 188: 3290-3298 [Abstract] [Full Text]  
• Dominguez-Cuevas, P., Gonzalez-Pastor, J.-E., Marques, S., Ramos, J.-L., de Lorenzo, V. (2006). Transcriptional Tradeoff between Metabolic and Stress-response Programs in Pseudomonas putida KT2440 Cells Exposed to Toluene. J. Biol. Chem. 281: 11981-11991 [Abstract] [Full Text]  
• Segura, A., Godoy, P., van Dillewijn, P., Hurtado, A., Arroyo, N., Santacruz, S., Ramos, J.-L. (2005). Proteomic Analysis Reveals the Participation of Energy- and Stress-Related Proteins in the Response of Pseudomonas putida DOT-T1E to Toluene. J. Bacteriol. 187: 5937-5945 [Abstract] [Full Text]  
• Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  
• Guazzaroni, M.-E., Krell, T., Felipe, A., Ruiz, R., Meng, C., Zhang, X., Gallegos, M.-T., Ramos, J. L. (2005). The Multidrug Efflux Regulator TtgV Recognizes a Wide Range of Structurally Different Effectors in Solution and Complexed with Target DNA: EVIDENCE FROM ISOTHERMAL TITRATION CALORIMETRY. J. Biol. Chem. 280: 20887-20893 [Abstract] [Full Text]  
• Chatterjee, A., Chaudhuri, S., Saha, G., Gupta, S., Chowdhury, R. (2004). Effect of Bile on the Cell Surface Permeability Barrier and Efflux System of Vibrio cholerae. J. Bacteriol. 186: 6809-6814 [Abstract] [Full Text]  
• Rojas, A., Duque, E., Schmid, A., Hurtado, A., Ramos, J.-L., Segura, A. (2004). Biotransformation in Double-Phase Systems: Physiological Responses of Pseudomonas putida DOT-T1E to a Double Phase Made of Aliphatic Alcohols and Biosynthesis of Substituted Catechols. Appl. Environ. Microbiol. 70: 3637-3643 [Abstract] [Full Text]  
• Guazzaroni, M.-E., Teran, W., Zhang, X., Gallegos, M.-T., Ramos, J. L. (2004). TtgV Bound to a Complex Operator Site Represses Transcription of the Promoter for the Multidrug and Solvent Extrusion TtgGHI Pump. J. Bacteriol. 186: 2921-2927 [Abstract] [Full Text]  
• Teran, W., Felipe, A., Segura, A., Rojas, A., Ramos, J.-L., Gallegos, M.-T. (2003). Antibiotic-Dependent Induction of Pseudomonas putida DOT-T1E TtgABC Efflux Pump Is Mediated by the Drug Binding Repressor TtgR. Antimicrob. Agents Chemother. 47: 3067-3072 [Abstract] [Full Text]