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Journal of Bacteriology, August 2003, p. 4883-4890, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4883-4890.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Regulation of the Bacillus subtilis Extracytoplasmic Function Protein {sigma}Y and Its Target Promoters

Min Cao,1 Letal Salzberg,1 Ching Sung Tsai,1 Thorsten Mascher,1 Carla Bonilla,2 Tao Wang,3 Rick W. Ye,3 Leticia Márquez-Magaña,2 and John D. Helmann1*

Department of Microbiology, Cornell University, Ithaca, New York 14853-8101,1 Department of Biology, San Francisco State University, San Francisco, California 94132,2 Experimental Station E328/148B, DuPont Central Research and Development, Wilmington, Delaware 198823

Received 3 April 2003/ Accepted 20 May 2003

The Bacillus subtilis extracytoplasmic function sigma factor {sigma}Y is of unknown function. We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, PY. We selected for transposon-induced mutations that upregulate PY transcription in an attempt to identify genes involved in {sigma}Y regulation. The resulting insertions disrupted yxlC, the gene immediately downstream of sigY. However, the phenotype of the yxlC::Tn10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of PY. Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of {sigma}Y activity. A comparison of the transcriptomes of wild-type and yxlC::Tn10 mutant strains revealed elevated expression of several operons. However, only one additional gene, ybgB, was unambiguously identified as a direct target for {sigma}Y. This was supported by analysis of direct targets for {sigma}Y transcription with whole-genome runoff transcription followed by macroarray analysis.

* Corresponding author. Mailing address: Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-6570. Fax: (607) 255-3904. E-mail: jdh9{at}cornell.edu.

Journal of Bacteriology, August 2003, p. 4883-4890, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4883-4890.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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