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Journal of Bacteriology, August 2003, p. 4983-4991, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4983-4991.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of a Regulated Alkaline Phosphatase, a Cell Surface-Associated Lipoprotein, in Mycobacterium smegmatis

Jordan Kriakov, Sun hee Lee, and William R. Jacobs Jr.*

Department of Microbiology and Immunology, Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, New York 10461

Received 26 February 2003/ Accepted 27 May 2003

Although alkaline phosphatases are common in a wide variety of bacteria, there has been no prior evidence for alkaline phosphatases in Mycobacterium smegmatis. Here we report that transposon insertions in the pst operon, encoding homologues of an inorganic phosphate transporter, leads to constitutive expression of a protein with alkaline phosphatase activity. DNA sequence analysis revealed that M. smegmatis does indeed have a phoA gene that shows high homology to other phoA genes. The M. smegmatis phoA gene was shown to be induced by phosphate starvation and thus negatively regulated by the pst operon. Interestingly, the putative M. smegmatis PhoA has a hydrophobic N-terminal domain which resembles a lipoprotein signal sequence. The M. smegmatis PhoA was demonstrated to be an exported protein associated with the cell surface. Furthermore, immunoprecipitation of PhoA from [14C]acetate-labeled M. smegmatis cell lysates demonstrated that this phosphatase is a lipoprotein.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461. Phone: (718) 430-2888. Fax: (718) 518-0366. E-mail: Jacobsw{at}hhmi.org.

Journal of Bacteriology, August 2003, p. 4983-4991, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.4983-4991.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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