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The Product of the fimI Gene Is Necessary for Escherichia coli Type 1 Pilus Biosynthesis

College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606¹

Received 18 April 2003/ Accepted 20 May 2003

Site-directed mutagenesis was employed to create lesions in fimI, a gene of uncertain function located in the chromosomal gene cluster (fim) involved in Escherichia coli type 1 pilus biosynthesis. Chromosomal fimI mutations produced a piliation-negative phenotype. Complementation analysis indicated that a fimI'-kan insertion mutation and a fimI frameshift mutation produced polarity-like effects not seen with an in-frame fimI deletion mutation. Minicell analysis associated fimI with a 16.4-kDa noncytoplasmic protein product (FimI). We conclude that FimI has a required role in normal pilus biosynthesis.

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