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Journal of Bacteriology, August 2003, p. 5012-5014, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.5012-5014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Regulated Expression of the Escherichia coli dam Gene

Melissa A. Calmann and M. G. Marinus*

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 2 April 2003/ Accepted 23 May 2003

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

* Corresponding author. Mailing address: Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Ave., Worcester, MA 01655. Phone: (508) 856-3330. Fax: (508) 856-3036. E-mail: martin.marinus{at}umassmed.edu.

Journal of Bacteriology, August 2003, p. 5012-5014, Vol. 185, No. 16
0021-9193/03/$08.00+0     DOI: 10.1128/JB.185.16.5012-5014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nicoloff, H., Perreten, V., Levy, S. B. (2007). Increased Genome Instability in Escherichia coli lon Mutants: Relation to Emergence of Multiple-Antibiotic-Resistant (Mar) Mutants Caused by Insertion Sequence Elements and Large Tandem Genomic Amplifications. Antimicrob. Agents Chemother. 51: 1293-1303 [Abstract] [Full Text]  
  • Erova, T. E., Pillai, L., Fadl, A. A., Sha, J., Wang, S., Galindo, C. L., Chopra, A. K. (2006). DNA Adenine Methyltransferase Influences the Virulence of Aeromonas hydrophila. Infect. Immun. 74: 410-424 [Abstract] [Full Text]  


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